Grafted Neural Precursors Integrate Into Mouse Striatum, Differentiate and Promote Recovery of Function Through Release of Erythropoietin in MPTP-Treated Mice
| Autor: | Stephana Carelli1,, Giallongo, Toniella, Viaggi, Cristina, Gombalova, Zuzana, Latorre, Elisa, Mazza, Massimiliano, Vaglini, Francesca, Giulio, Anna Maria Di, Gorio, Alfredo |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Tyrosine 3-Monooxygenase adult neural stem cells Cell- and Tissue-Based Therapy regenerative medicine Mice Transgenic Nerve Tissue Proteins Choline O-Acetyltransferase lcsh:RC321-571 Mice Neural Stem Cells Antigens Neoplasm otorhinolaryngologic diseases Animals Muscle Strength Antigens Erythropoietin lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry MPTP gamma-Aminobutyric Acid Movement Disorders MPTP Poisoning Recovery of Function Corpus Striatum Mice Inbred C57BL Disease Models Animal stomatognathic diseases Parkinson’s disease Original Article Proteoglycans cell therapy |
| Zdroj: | ASN Neuro, Vol 8 (2016) ASN NEURO |
| ISSN: | 1759-0914 |
| Popis: | Erythropoietin-releasing neural precursor cells (Er-NPCs) are a subclass of subventricular zone-derived neural progenitors, capable of surviving for 6 hr after death of donor. They present higher neural differentiation. Here, Er-NPCs were studied in animal model of Parkinson’s disease. Dopaminergic degeneration was caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intraperitoneal administration in C57BL/6 mice. The loss of function was evaluated by specific behavioral tests. Er-NPCs (2.5 × 10 5 ) expressing the green fluorescent protein were administered by stereotaxic injection unilaterally in the left striatum. At the end of observational research period (2 weeks), most of the transplanted Er-NPCs were located in the striatum, while several had migrated ventrally and caudally from the injection site, up to ipsilateral and contralateral substantia nigra. Most of transplanted cells had differentiated into dopaminergic, cholinergic, or GABAergic neurons. Er-NPCs administration also promoted a rapid functional improvement that was already evident at the third day after cells administration. This was accompanied by enhanced survival of nigral neurons. These effects were likely promoted by Er-NPCs-released erythropoietin (EPO), since the injection of Er-NPCs in association with anti-EPO or anti-EPOR antibodies had completely neutralized the recovery of function. In addition, intrastriatal administration of recombinant EPO mimics the effects of Er-NPCs. We suggest that Er-NPCs, and cells with similar properties, may represent good candidates for cellular therapy in neurodegenerative disorders of this kind. |
| Databáze: | OpenAIRE |
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