Distinct Patterns of Colocalization of the CCND1 and CMYC Genes With Their Potential Translocation Partner IGH at Successive Stages of B-Cell Differentiation

Autor: Ilya, Sklyar, Olga V, Iarovaia, Alexey A, Gavrilov, Andrey, Pichugin, Diego, Germini, Tatiana, Tsfasman, Gersende, Caron, Thierry, Fest, Marc, Lipinski, Sergey V, Razin, Yegor S, Vassetzky
Přispěvatelé: Institute of Gene Biology, Russian Academy of Sciences, Interactions moléculaires et cancer (IMC (UMR 8126)), Signalisation, noyaux et innovations en cancérologie (UMR8126), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Peter the Great St. Petersburg Polytechnic University (SPbPU), Pôle biologie, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Etablissement français du sang [Rennes] (EFS Bretagne), Microenvironnement et cancer (MiCa), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Faculty of biology, Lomonosov Moscow State University (MSU), This research was supported by the Russian Science Foundation, project #14-24-00022 to IS, OVI, AAG and SVR and by grants from INCa (ERABL) and ANRS (#1154) to YSV. AG is a fellow of Dmitri Zimin’s Dynasty Foundation., Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Jonchère, Laurent
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Journal of Cellular Biochemistry
Journal of Cellular Biochemistry, Wiley, 2016, 117 (7), pp.1506-1510. ⟨10.1002/jcb.25516⟩
Journal of Cellular Biochemistry, 2016, 117 (7), pp.1506-1510. ⟨10.1002/jcb.25516⟩
ISSN: 0730-2312
1097-4644
DOI: 10.1002/jcb.25516⟩
Popis: The immunoglobulin heavy chain (IGH) locus is submitted to intra-chromosomal DNA breakages and rearrangements during normal B cell differentiation that create a risk for illegitimate inter-chromosomal translocations leading to a variety of B-cell malignancies. In most Burkitt's and Mantle Cell lymphomas, specific chromosomal translocations juxtapose the IGH locus with a CMYC or Cyclin D1 (CCND1) gene, respectively. 3D-fluorescence in situ hybridization was performed on normal peripheral B lymphocytes induced to mature in vitro from a naive state to the stage where they undergo somatic hypermutation (SHM) and class switch recombination (CSR). The CCND1 genes were found very close to the IGH locus in naive B cells and further away after maturation. In contrast, the CMYC alleles became localized closer to an IGH locus at the stage of SHM/CSR. The colocalization observed between the two oncogenes and the IGH locus at successive stages of B-cell differentiation occurred in the immediate vicinity of the nucleolus, consistent with the known localization of the RAGs and AID enzymes whose function has been demonstrated in IGH physiological rearrangements. We propose that the chromosomal events leading to Mantle Cell lymphoma and Burkitt's lymphoma are favored by the colocalization of CCND1 and CMYC with IGH at the time the concerned B cells undergo VDJ recombination or SHM/CSR, respectively. J. Cell. Biochem. 117: 1506-1510, 2016. © 2016 Wiley Periodicals, Inc.
Databáze: OpenAIRE
Externí odkaz: