Identification and characterization of novel factors that act in the nonsense‐mediated mRNA decay pathway in nematodes, flies and mammals
| Autor: | Casadio, Angela, Longman, Dasa, Hug, Nele, Delavaine, Laurent, Vallejos Baier, Raúl, Alonso, Claudio R, Cáceres, Javier F |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
nonsense-mediated decay
Embryo Nonmammalian Recombinant Fusion Proteins Scientific Reports Egg Proteins Microfilament Proteins Nuclear Proteins smg genes C. elegans Nonsense Mediated mRNA Decay Animals Genetically Modified Evolution Molecular Drosophila melanogaster GTP-Binding Proteins Gene Knockdown Techniques QH0426 Animals Drosophila Proteins Humans RNA Interference Caenorhabditis elegans Caenorhabditis elegans Proteins Carrier Proteins RNAi screen HeLa Cells |
| Zdroj: | Casadio, A, Longman, D, Hug, N, Delavaine, L, Vallejos Baier, R, Alonso, C R & Cáceres, J F 2015, ' Identification and characterization of novel factors that act in the nonsense-mediated mRNA decay pathway in nematodes, flies and mammals ', EMBO Reports, vol. 16, no. 1, pp. 71-78 . https://doi.org/10.15252/embr.201439183 EMBO Reports |
| ISSN: | 1469-221X |
| Popis: | Nonsense‐mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome‐wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD genes that are conserved throughout evolution. Two of their human homologs, GNL2 (ngp‐1) and SEC13 (npp‐20), are also required for NMD in human cells. We also show that the C. elegans gene noah‐2, which is present in Drosophila melanogaster but absent in humans, is an NMD factor in fruit flies. Altogether, these data identify novel NMD factors that are conserved throughout evolution, highlighting the complexity of the NMD pathway and suggesting that yet uncovered novel factors may act to regulate this process. |
| Databáze: | OpenAIRE |
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