In Vivo Profiling Reveals a Competent Heat Shock Response in Adult Neurons: Implications for Neurodegenerative Disorders
| Autor: | Carnemolla, Alisia, Lazell, Hayley, Moussaoui, Saliha, Bates, Gillian P. |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Chromosomes Artificial Bacterial Pyridones Administration Oral lcsh:Medicine Mice Transgenic Hippocampus Mice Animals HSP90 Heat-Shock Proteins RNA Messenger lcsh:Science Neurons lcsh:R Brain Neurodegenerative Diseases Flow Cytometry Corpus Striatum Oligodendroglia Pyrimidines Gene Expression Regulation Astrocytes Female lcsh:Q Heat-Shock Response Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 7, p e0131985 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The heat shock response (HSR) is the main pathway used by cells to counteract proteotoxicity. The inability of differentiated neurons to induce an HSR has been documented in primary neuronal cultures and has been proposed to play a critical role in ageing and neurodegeneration. However, this accepted dogma has not been demonstrated in vivo. We used BAC transgenic mice generated by the Gene Expression Nervous System Atlas project to investigate the capacity of striatal medium sized spiny neurons to induce an HSR as compared to that of astrocytes and oligodendrocytes. We found that all cell populations were competent to induce an HSR upon HSP90 inhibition. We also show the presence and relative abundance of heat shock-related genes and proteins in these striatal cell populations. The identification of a competent HSR in adult neurons supports the development of therapeutics that target the HSR pathway as treatments for neurodegenerative disorders. |
| Databáze: | OpenAIRE |
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