Abnormal glycosylation of hemopexin in arthritic rats can be blocked by bindarit
| Autor: | luciano saso, Silvestrini, B., Zwain, I., Guglielmotti, A., Luparini, M. R., Cioli, V., Cheng, C. Y. |
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| Rok vydání: | 1992 |
| Předmět: |
Glycosylation
Indazoles Staining and Labeling Freund's Adjuvant Immunoblotting Molecular Sequence Data Blood Proteins Arthritis Experimental Rats Rats Sprague-Dawley Hemopexin Endopeptidases Concanavalin A Animals Electrophoresis Polyacrylamide Gel Amino Acid Sequence Propionates Acute-Phase Reaction Chromatography High Pressure Liquid |
| Zdroj: | Scopus-Elsevier |
| Popis: | Induction of arthritis in rats with Freund's complete adjuvant was accompanied by a distinctive alteration of concanavalin A (Con-A) reactivity in their serum proteins in which the concentrations of selected Con-A reactive proteins were significantly higher when compared to healthy rats. To assess if the observed increase in Con-A reactivity of specific serum proteins reflects an increase in carbohydrate moieties in these proteins in addition to an increase in their protein concentrations, a heme binding serum glycoprotein, hemopexin, also an acute phase reactant, was selected as a marker protein. Hemopexin was purified to apparent homogeneity from pools of serum samples derived from rats with yeast induced inflammation, a monospecific polyclonal antibody was prepared and was used for immunoblot analysis. It was noted that the concentration of hemopexin increased in rats with adjuvant induced arthritis; however, its concentration fell to normal levels after administration with a newly synthesized drug, bindarit, (2-[(1-benzyl-indazol-3-yl)methoxy]-2-methyl propionic acid, C19H20N2O3. Hemopexin was micropurified individually from healthy rats, adjuvant induced arthritic rats, and adjuvant arthritic rats treated with bindarit, cleaved with a Glu-C endopeptidase, Staphylococcus aureus protease V8, and the resultant peptide fragments resolved by SDS-PAGE and examined by silver staining, Coomassie blue staining, and lectin blots using Con-A. It was subsequently noted that hemopexin isolated from adjuvant induced arthritic rats showed a significant increase in Con-A reactivity in selected peptide fragments and that such an increase in glycosylation could be reversed to a pattern similar to healthy rats following treatment with bindarit.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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