Metformin Attenuates Postinfarction Myocardial Fibrosis and Inflammation in Mice

Autor: Halyna Loi, Solomiia Kramar, Charlotte Laborde, Dimitri Marsal, Nathalie Pizzinat, Daniel Cussac, Jerome Roncalli, Frederic Boal, Helene Tronchere, Oleksandra Oleshchuk, Mykhaylo Korda, Oksana Kunduzova
Přispěvatelé: I.Horbachevsky Ternopil State Medical University, Ternopil, Ukraine, Institut des Maladies Métaboliques et Casdiovasculaires (UPS/Inserm U1297 - I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, pizzinat, Nathalie
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, MDPI, 2021, 22 (17), pp.9393. ⟨10.3390/ijms22179393⟩
Volume 22
Issue 17
International Journal of Molecular Sciences, 2021, 22 (17), pp.9393. ⟨10.3390/ijms22179393⟩
International Journal of Molecular Sciences, Vol 22, Iss 9393, p 9393 (2021)
ISSN: 1661-6596
1422-0067
DOI: 10.3390/ijms22179393⟩
Popis: International audience; Diabetes is a major risk factor for the development of cardiovascular disease with a higher incidence of myocardial infarction. This study explores the role of metformin, a first-line antihyperglycemic agent, in postinfarction fibrotic and inflammatory remodeling in mice. Three-month-old C57BI/6J mice were submitted to 30 min cardiac ischemia followed by reperfusion for 14 days. Intraperitoneal treatment with metformin (5 mg/kg) was initiated 15 min after the onset of reperfusion and maintained for 14 days. Real-time PCR was used to determine the levels of COL3A1, αSMA, CD68, TNF-α and IL-6. Increased collagen deposition and infiltration of macrophages in heart tissues are associated with upregulation of the inflammation-associated genes in mice after 14 days of reperfusion. Metformin treatment markedly reduced postinfarction fibrotic remodeling and CD68-positive cell population in mice. Moreover, metformin resulted in reduced expression of COL3A1, αSMA and CD68 after 14 days of reperfusion. Taken together, these results open new perspectives for the use of metformin as a drug that counteracts adverse myocardial fibroticand inflammatory remodeling after MI.
Databáze: OpenAIRE
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