Comparative analysis of Alzheimer’s disease Cerebrospinal fluid biomarkers measurement by multiplex SOMAscan platform and immunoassay-based approach
| Autor: | Timsina, Jigyasha, Gomez-Fonseca, Duber, Xiong, Chengjie, Schindler, Suzanne E, Fagan, Anne M, Bateman, Randall J, Farlow, Martin, Morris, John C, Perrin, Richard J, Moulder, Krista, Hassenstab, Jason, Vöglein, Jonathan, Wang, Lihua, Chhatwal, Jasmeer, Mori, Hiroshi, Initiative, Alzheimer’s Disease Neuroimaging, Consortia, Dominantly Inherited Alzheimer Network, Sung, Yun Ju, Cruchaga, Carlos, Do, Anh, Western, Dan, Alvarez, Ignacio, Aguilar, Miquel, Pastor, Pau, Henson, Rachel L, Herries, Elizabeth |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Immunoassay
Amyloid beta-Peptides assays diagnosis [Alzheimer Disease] tau Proteins cerebrospinal fluid [Amyloid beta-Peptides] Article cerebrospinal fluid [Alzheimer Disease] cerebrospinal fluid [Biomarkers] cerebrospinal fluid [tau Proteins] ROC Curve Alzheimer Disease correlation Humans Neurogranin ddc:610 cerebrospinal fluid [Neurogranin] SOMAscan cerebrospinal fluid biomarkers Alzheimer’s disease Biomarkers |
| Zdroj: | J Alzheimers Dis Journal of Alzheimer's disease 89(1), 193-207 (2022). doi:10.3233/JAD-220399 |
| DOI: | 10.3233/JAD-220399 |
| Popis: | BACKGROUND: The SOMAscan assay has an advantage over immunoassay-based methods because it measures a large number of proteins in a cost-effective manner. However, the performance of this technology compared to the routinely used immunoassay techniques needs to be evaluated. OBJECTIVE: We performed comparative analyses of SOMAscan and immunoassay-based protein measurements for five cerebrospinal fluid (CSF) proteins associated with Alzheimer’s disease (AD) and neurodegeration: NfL, Neurogranin, sTREM2, VILIP-1 and SNAP-25. METHODS: We compared biomarkers measured in ADNI (N=689), Knight-ADRC (N=870), DIAN (N=115), and Barcelona-1 (N=92) cohorts. Raw protein values were transformed using z-score in order to combine measures from the different studies. sTREM2 and VILIP-1 had more than one analyte in SOMAscan; all available analytes were evaluated. Pearson’s correlation coefficients between SOMAscan and immunoassays were calculated. Receiver operating characteristic curve and area under the curve were used to compare prediction accuracy of these biomarkers between the two platforms. RESULTS: Neurogranin, VILIP-1 and NfL showed high correlation between SOMAscan and immunoassay measures (r > 0.9). sTREM2 had a fair correlation (r > 0.6), whereas SNAP-25 showed weak correlation (r = 0.06). Measures in both platforms provided similar predicted performance for all biomarkers except SNAP-25 and one of the sTREM2 analytes. sTREM2 showed higher AUC for SOMAscan based measures. CONCLUSION: Our data indicate that SOMAscan performs as well as immunoassay approaches for NfL, Neurogranin, VILIP-1 and sTREM2. Our study shows promise for using SOMAscan as an alternative to traditional immunoassay-based measures. Follow-up investigation will be required for SNAP-25 and additional established biomarkers. |
| Databáze: | OpenAIRE |
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