Leptin expression in the ovine mammary gland : putative sequential involvement of adipose, epithelial and myoepithelial cells during pregnancy and lactation
| Autor: | Muriel Bonnet, Isabelle Gourdou, Leroux, C., Yves Chilliard, Jean Djiane |
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| Přispěvatelé: | Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA), Unité de biologie cellulaire et moléculaire, Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Leptin
DNA Complementary Sheep Time Factors Base Sequence Reverse Transcriptase Polymerase Chain Reaction [SDV]Life Sciences [q-bio] Molecular Sequence Data Epithelial Cells [INFO] Computer Science [cs] Immunohistochemistry [SDV] Life Sciences [q-bio] Mammary Glands Animal Pregnancy Adipocytes Animals Lactation Female [INFO]Computer Science [cs] RNA Messenger ComputingMilieux_MISCELLANEOUS |
| Zdroj: | Journal of Animal Science Journal of Animal Science, American Society of Animal Science, 2002, 80, pp.723-728 HAL |
| ISSN: | 0021-8812 1525-3163 |
| Popis: | We examined the ability of the ovine mammary gland to synthesize leptin throughout pregnancy and lactation. Leptin gene expression was assayed by real-time reverse transcription and polymerase chain reaction in mammary gland from ewes at 15, 80, 106, 112, or 141 d of pregnancy and at 0 (30 min after parturition), 3, 48, or 70 d of lactation. Leptin mRNA level was high at the beginning (the first 80 d) and at the end of pregnancy and was lower at mid-pregnancy and throughout lactation. Furthermore, during these periods of mammary leptin expression, the location of leptin protein, as determined by immunohistochemical analysis, changed within mammary tissue. It was located in adipose cells during early stages of pregnancy, in epithelial cells after full cell differentiation just before parturition, and in myoepithelial cells after parturition. These data, compared with published data on leptin receptor gene expression, provide evidence that leptin could be produced by different cell types of the mammary gland and could act as a paracrine factor on mammary cell growth and differentiation via adipose-epithelial cells and myoepithelial-epithelial cell interactions. |
| Databáze: | OpenAIRE |
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