Coxsackie adenovirus receptor and alpha nu beta3/alpha nu beta5 integrins in adenovirus gene transfer of rat cochlea
| Autor: | Venail, Frédéric, Wang, Jing, Ruel, Jérôme, Ballana, Ester, Rebillard, Guy, Eybalin, Michel, Arbones, M., Bosch, A., Puel, Jean-Luc |
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| Přispěvatelé: | Physiopathologie et thérapie des déficits sensoriels et moteurs, Université Montpellier 2 - Sciences et Techniques (UM2)-IFR76-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Anatomie [CHU Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Genes and Disease Program, Centre de Regulacio´ Geno´mica-CRG-UPF, Department of Biochemistry and Molecular Biology, Centre of Animal Biotechnology and Gene Therapy (CBATEG), Universitat Autònoma de Barcelona (UAB), Hamel, Christian |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Integrin beta3
Integrins Integrin beta Chains Action Potentials Gene Expression MESH: Microscopy Fluorescence Integrin alpha5 Tissue Culture Techniques Transduction Genetic MESH: Genetic Vectors MESH: Cochlea MESH: Integrin beta Chains MESH: Animals Transgenes MESH: Action Potentials Integrin beta3 MESH: Adenoviridae MESH: Integrins MESH: Transduction Genetic Immunohistochemistry Cochlea MESH: Models Animal Models Animal Receptors Virus [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Integrin alpha5 Coxsackie and Adenovirus Receptor-Like Membrane Protein MESH: Gene Expression MESH: Rats Genetic Vectors Green Fluorescent Proteins MESH: Transgenes Adenoviridae Injections MESH: Green Fluorescent Proteins otorhinolaryngologic diseases Animals Humans MESH: Injections [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Tissue Culture Techniques Rats Wistar Cochlear Nerve MESH: Cochlear Nerve MESH: Humans MESH: Immunohistochemistry Genetic Therapy MESH: Rats Wistar MESH: Receptors Virus Rats Microscopy Fluorescence MESH: Gene Therapy |
| Zdroj: | Gene Therapy Gene Therapy, Nature Publishing Group, 2007, 14 (1), pp.30-7. ⟨10.1038/sj.gt.3302826⟩ |
| ISSN: | 0969-7128 1476-5462 |
| DOI: | 10.1038/sj.gt.3302826⟩ |
| Popis: | International audience; This study was designed to determine whether Coxsackie adenovirus receptor (CAR) and alpha nu beta3/alpha nu beta5 integrin co-receptors are involved in adenovirus gene transfer in the rat cochlea. We find that CAR and integrin co-receptors are expressed in every cell subtype transduced by the adenoviral vector Ad5 DeltaE1-E3/cytomegalovirus/green fluorescent protein (GFP) on cochlear slices in vitro. The spiral ganglion neurons, which do not express CAR, were not transduced by the virus. Blocking these receptors by monoclonal antibodies decreased transgene expression, whereas disrupting tight junctions with ethylenediaminetetraacetic acid led to an increased transgene expression. However, sensory hair cells and strial cells also expressing CAR and alpha nu integrins were not transduced by the vector. GFP expression was also studied in vivo. Perilymphatic perfusion of adenovirus in vivo did not affect hearing and only cells lining the perilymphatic spaces were transduced. Endolymphatic perfusion resulted in low-frequency hearing loss and although some cells of the organ of Corti were efficiently transduced, the sensory and the strial cells were not. Transduced sensory and strial cells were occasionally observed in cochleas after single shot of adenovirus. Pretreatment with anti-CAR and anti-alpha nu antibodies decreases GFP expression in vivo, suggesting that the CAR/alpha nu integrin pathway is involved in adenovirus transduction in the cochlea. |
| Databáze: | OpenAIRE |
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