The brain as immunoprecipitator of serum autoantibodies against N-Methyl-D-aspartate receptor subunit NR1
| Autor: | Castillo-Gomez, E., Kästner, A., Steiner, J., Schneider, A., Hettling, B., Poggi, G., Ostehr, K., Uhr, M., Asif, A., Matzke, M., Schmidt, U., Pfander, V., Hammer, C., Schulz, T., Binder, L., Stöcker, W., Weber, F., Ehrenreich, H. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Multiple Sclerosis Brain Middle Aged Receptors N-Methyl-D-Aspartate blood [Multiple Sclerosis] Mice Inbred C57BL Mice cerebrospinal fluid [Autoantibodies] Blood-Brain Barrier immunology [Receptors N-Methyl-D-Aspartate] Seroepidemiologic Studies Animals Humans blood [Autoantibodies] immunology [Brain] Female ddc:610 NR1 NMDA receptor physiopathology [Blood-Brain Barrier] cerebrospinal fluid [Multiple Sclerosis] Autoantibodies |
| Zdroj: | Annals of neurology 79(1), 144-151 (2015). doi:10.1002/ana.24545 Annals of Neurology |
| DOI: | 10.1002/ana.24545 |
| Popis: | Autoantibodies (AB) against N-methyl-D-aspartate receptor subunit NR1 (NMDAR1) are highly seroprevalent in health and disease. Symptomatic relevance may arise upon compromised blood-brain barrier (BBB). However, it remained unknown whether circulating NMDAR1 AB appear in the cerebrospinal fluid (CSF). Of n = 271 subjects with CSF-serum pairs, 26 were NMDAR1 AB seropositive, but only 1 was CSF positive. Contrariwise, tetanus AB (non-brain-binding) were present in serum and CSF of all subjects, with CSF levels higher upon BBB dysfunction. Translational mouse experiments proved the hypothesis that the brain acts as an 'immunoprecipitator'; simultaneous injection of NMDAR1 AB and the non-brain-binding green fluorescent protein AB resulted in high detectability of the former in brain and the latter in CSF. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|