Salivary and serum anti-desmoglein 1 and 3 ELISA and indirect immunofluorescence in pemphigus vulgaris: Correlations with serum elisa, indirect immunofluorescence and disease severity
| Autor: | Maryam Koopaie, Mortazavi, H., Khatami, A., Khodashenas, Z. |
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| Předmět: |
autoimmune blistering diseases
desmoglein1 desmoglein3 pemphigus vulgaris Adult Male Desmoglein 3 Desmoglein 1 Enzyme-Linked Immunosorbent Assay Middle Aged Severity of Illness Index Young Adult Cross-Sectional Studies Predictive Value of Tests Humans Female Fluorescent Antibody Technique Indirect Saliva Pemphigus Aged Autoantibodies |
| Zdroj: | Scopus-Elsevier Acta Dermatovenerologica Croatica Volume 26 Issue 2 |
| ISSN: | 1330-027X 1847-6538 |
| Popis: | Anti-desmoglein (anti-Dsg) ELISA and indirect immunofluorescence (IIF) are used for the diagnosis of pemphigus vulgaris (PV). The value of salivary ELISA, serum ELISA, and IIF in the diagnosis of PV, and the correlation of salivary anti-Dsg1 and anti-Dsg3 ELISA with serum ELISA, serum and salivary IIF titers, and disease severity in patients with PV were evaluated. Fifty newly diagnosed patients with PV were enrolled in the study. Demographic data and disease-severity scores were recorded for each patient. Anti-Dsg1 and anti-Dsg3 ELISA and IIF were performed on both serum and salivary samples. Given the cut-off value of 20 RU/mL for Dsg1 and Dsg3, serum Dsg1 and Dsg3 ELISA were positive in 41 (82%) and 40 (80%) patients, and saliva Dsg1 and the Dsg3 ELISA were positive in 2 (4%) and 3 (6%) patients, respectively. Using the cut-off value of 13.4 RU/mL and 7.7 RU/mL for Dsg3 and Dsg1 salivary ELISA, 25 (50%) and 23 (46%) patients tested positive for Dsg3 and Dsg1, respectively. Serum IIF results were positive in 35 (70%) patients, and salivary IIF results were positive in 16 (32%) patients. Salivary anti-Dsg1 and anti-Dsg3 showed moderate correlations with the total pemphigus disease area index (PDAI) score (r=0.466, P |
| Databáze: | OpenAIRE |
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