Low-dose exposure to bisphenols A, F and S of human primary adipocyte impacts coding and non-coding RNA profiles
Autor: | Verbanck, Marie, Canouil, Mickaël, Leloire, Audrey, Dhennin, Véronique, Coumoul, Xavier, Yengo, Loïc, Froguel, Philippe, Poulain-Godefroy, Odile |
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Přispěvatelé: | Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (EGENODIA (GI3M)), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université Paris Cité (UPCité), Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire (T3S - UMR_S 1124), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Dissemin, Projet, Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (GI3M), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Paris (UP), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
RNA
Untranslated Databases Factual Microarrays [SDV]Life Sciences [q-bio] lcsh:Medicine Endocrine Disruptors Biochemistry Animal Cells Adipocytes Medicine and Health Sciences Sulfones Small nucleolar RNAs lcsh:Science Cells Cultured Connective Tissue Cells Cell Differentiation dissemin Up-Regulation Nucleic acids [SDV] Life Sciences [q-bio] Chemistry Bioassays and Physiological Analysis Connective Tissue Physical Sciences Cellular Types Anatomy hormones hormone substitutes and hormone antagonists Research Article Pollutants endocrine system Down-Regulation Research and Analysis Methods Phenols Genetics Adipocyte Differentiation Humans Environmental Chemistry RNA Messenger Benzhydryl Compounds Non-coding RNA Biology and life sciences urogenital system lcsh:R Ecology and Environmental Sciences Cell Biology Gene regulation MicroRNAs Biological Tissue RNA lcsh:Q Gene expression Developmental Biology |
Zdroj: | PLoS ONE PLoS ONE, 2017, 12 (6), pp.e0179583. ⟨10.1371/journal.pone.0179583⟩ PLoS ONE, Vol 12, Iss 6, p e0179583 (2017) PLoS ONE, Public Library of Science, 2017, 12 (6), pp.e0179583. ⟨10.1371/journal.pone.0179583⟩ |
ISSN: | 1932-6203 |
Popis: | International audience; Bisphenol A (BPA) exposure has been suspected to be associated with deleterious effects on health including obesity and metabolically-linked diseases. Although bisphenols F (BPF) and S (BPS) are BPA structural analogs commonly used in many marketed products as a replacement for BPA, only sparse toxicological data are available yet. Our objective was to comprehensively characterize bisphenols gene targets in a human primary adipocyte model, in order to determine whether they may induce cellular dysfunction, using chronic exposure at two concentrations: a “low-dose” similar to the dose usually encountered in human biological fluids and a higher dose. Therefore, BPA, BPF and BPS have been added at 10 nM or 10 μM during the differentiation of human primary adipocytes from subcutaneous fat of three non-diabetic Caucasian female patients. Gene expression (mRNA/lncRNA) arrays and microRNA arrays, have been used to assess coding and non-coding RNA changes. We detected significantly deregulated mRNA/lncRNA and miRNA at low and high doses. Enrichment in “cancer” and “organismal injury and abnormalities” related pathways was found in response to the three products. Some long intergenic non-coding RNAs and small nucleolar RNAs were differentially expressed suggesting that bisphenols may also activate multiple cellular processes and epigenetic modifications. The analysis of upstream regulators of deregulated genes highlighted hormones or hormone-like chemicals suggesting that BPS and BPF can be suspected to interfere, just like BPA, with hormonal regulation and have to be considered as endocrine disruptors. All these results suggest that as BPA, its substitutes BPS and BPF should be used with the same restrictions. |
Databáze: | OpenAIRE |
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