Low-dose exposure to bisphenols A, F and S of human primary adipocyte impacts coding and non-coding RNA profiles

Autor: Verbanck, Marie, Canouil, Mickaël, Leloire, Audrey, Dhennin, Véronique, Coumoul, Xavier, Yengo, Loïc, Froguel, Philippe, Poulain-Godefroy, Odile
Přispěvatelé: Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (EGENODIA (GI3M)), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université Paris Cité (UPCité), Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire (T3S - UMR_S 1124), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Dissemin, Projet, Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (GI3M), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Paris (UP), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
RNA
Untranslated
Databases
Factual
Microarrays
[SDV]Life Sciences [q-bio]
lcsh:Medicine
Endocrine Disruptors
Biochemistry
Animal Cells
Adipocytes
Medicine and Health Sciences
Sulfones
Small nucleolar RNAs
lcsh:Science
Cells
Cultured
Connective Tissue Cells
Cell Differentiation
dissemin
Up-Regulation
Nucleic acids
[SDV] Life Sciences [q-bio]
Chemistry
Bioassays and Physiological Analysis
Connective Tissue
Physical Sciences
Cellular Types
Anatomy
hormones
hormone substitutes
and hormone antagonists
Research Article
Pollutants
endocrine system
Down-Regulation
Research and Analysis Methods
Phenols
Genetics
Adipocyte Differentiation
Humans
Environmental Chemistry
RNA
Messenger
Benzhydryl Compounds
Non-coding RNA
Biology and life sciences
urogenital system
lcsh:R
Ecology and Environmental Sciences
Cell Biology
Gene regulation
MicroRNAs
Biological Tissue
RNA
lcsh:Q
Gene expression
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, 2017, 12 (6), pp.e0179583. ⟨10.1371/journal.pone.0179583⟩
PLoS ONE, Vol 12, Iss 6, p e0179583 (2017)
PLoS ONE, Public Library of Science, 2017, 12 (6), pp.e0179583. ⟨10.1371/journal.pone.0179583⟩
ISSN: 1932-6203
Popis: International audience; Bisphenol A (BPA) exposure has been suspected to be associated with deleterious effects on health including obesity and metabolically-linked diseases. Although bisphenols F (BPF) and S (BPS) are BPA structural analogs commonly used in many marketed products as a replacement for BPA, only sparse toxicological data are available yet. Our objective was to comprehensively characterize bisphenols gene targets in a human primary adipocyte model, in order to determine whether they may induce cellular dysfunction, using chronic exposure at two concentrations: a “low-dose” similar to the dose usually encountered in human biological fluids and a higher dose. Therefore, BPA, BPF and BPS have been added at 10 nM or 10 μM during the differentiation of human primary adipocytes from subcutaneous fat of three non-diabetic Caucasian female patients. Gene expression (mRNA/lncRNA) arrays and microRNA arrays, have been used to assess coding and non-coding RNA changes. We detected significantly deregulated mRNA/lncRNA and miRNA at low and high doses. Enrichment in “cancer” and “organismal injury and abnormalities” related pathways was found in response to the three products. Some long intergenic non-coding RNAs and small nucleolar RNAs were differentially expressed suggesting that bisphenols may also activate multiple cellular processes and epigenetic modifications. The analysis of upstream regulators of deregulated genes highlighted hormones or hormone-like chemicals suggesting that BPS and BPF can be suspected to interfere, just like BPA, with hormonal regulation and have to be considered as endocrine disruptors. All these results suggest that as BPA, its substitutes BPS and BPF should be used with the same restrictions.
Databáze: OpenAIRE
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