Nuclear morphology for the detection of alterations in bronchial cells from lung cancer: an attempt to improve sensitivity and specificity
| Autor: | Fafin-Lefevre, Mélanie, Morlais, Fabrice, Guittet, Lydia, Clin, Bénédicte, Launoy, Guy, Galateau-Sallé, Françoise, Plancoulaine, Benoît, Herlin, Paulette, Letourneux, Marc |
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| Přispěvatelé: | Groupe Régional d'Etudes sur le CANcer (GRECAN), IFR146-Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Cancers et Populations : Facteurs de Risque, Depistage, Pratiques Diagnostiques et Therapeutiques, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Santé au Travail et Pathologie Professionnellel [CHU Caen], Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Laboratoire d'Anatomie Pathologique [CHU Caen], Lefevre, Mélanie |
| Rok vydání: | 2011 |
| Předmět: |
MESH: Cell Nucleus
Lung Neoplasms [SDV.CAN]Life Sciences [q-bio]/Cancer Adenocarcinoma Sensitivity and Specificity MESH: Sputum [SDV.CAN] Life Sciences [q-bio]/Cancer Image Interpretation Computer-Assisted Humans bronchopulmonary cancer Aged MESH: Aged Cell Nucleus MESH: Humans MESH: Middle Aged screening MESH: Adenocarcinoma Sputum MESH: Carcinoma Squamous Cell Middle Aged cytometry MESH: Sensitivity and Specificity MESH: Lung Neoplasms MESH: Carcinoma Bronchogenic Carcinoma Bronchogenic Carcinoma Squamous Cell MESH: Image Interpretation Computer-Assisted |
| Zdroj: | Analytical and Quantitative Cytology and Histology Analytical and Quantitative Cytology and Histology, Journal of Reproductive Medicine, 2011, 33 (4), pp.183-95 |
| ISSN: | 0884-6812 |
| Popis: | International audience; OBJECTIVE: To identify which morphologic or densitometric parameters are modified in cell nuclei from bronchopulmonary cancer based on 18 parameters involving shape, intensity, chromatin, texture, and DNA content and develop a bronchopulmonary cancer screening method relying on analysis of sputum sample cell nuclei. STUDY DESIGN: A total of 25 sputum samples from controls and 22 bronchial aspiration samples from patients presenting with bronchopulmonary cancer who were professionally exposed to cancer were used. After Feulgen staining, 18 morphologic and DNA content parameters were measured on cell nuclei, via image cytom- etry. A method was developed for analyzing distribution quantiles, compared with simply interpreting mean values, to characterize morphologic modifications in cell nuclei. RESULTS: Distribution analysis of parameters enabled us to distinguish 13 of 18 parameters that demonstrated significant differences between controls and cancer cases. These parameters, used alone, enabled us to distinguish two population types, with both sensitivity and specificity > 70%. Three parameters offered 100% sensitivity and specificity. When mean values offered high sensitivity and specificity, comparable or higher sensitivity and specificity values were observed for at least one of the corresponding quantiles. CONCLUSION: Analysis of modification in morphologic parameters via distribution analysis proved promising for screening bronchopulmonary cancer from sputum. |
| Databáze: | OpenAIRE |
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