Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation
| Autor: | Goode, Debbie K., Obier, Nadine, Vijayabaskar, M.S., Lie-A-Ling, Michael, Lilly, Andrew J., Hannah, Rebecca, Lichtinger, Monika, Batta, Kiran, Florkowska, Magdalena, Patel, Rahima, Challinor, Mairi, Wallace, Kirstie, Gilmour, Jane, Assi, Salam A., Cauchy, Pierre, Hoogenkamp, Maarten, Westhead, David R., Lacaud, Georges, Kouskoff, Valerie, Göttgens, Berthold, Bonifer, Constanze |
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| Přispěvatelé: | Hannah, Rebecca [0000-0003-0477-7792], Gottgens, Berthold [0000-0001-6302-5705], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Resource
Homeodomain Proteins Manchester Cancer Research Centre Research Support Non-U.S. Gov't ResearchInstitutes_Networks_Beacons/mcrc Gene Expression Regulation Developmental Cell Differentiation Hematopoietic Stem Cells Hematopoiesis Mice Journal Article Animals Cell Lineage Gene Regulatory Networks Embryonic Stem Cells Developmental Biology Protein Binding Transcription Factors |
| Zdroj: | Developmental Cell Goode, D K, Obier, N, Vijayabaskar, M S, Lie-A-Ling, M, Lilly, A, Hannah, R, Lichtinger, M, Batta, K, Florkowska, M, Patel, R, Challinor, M, Wallace, K, Gilmour, J, Assi, S A, Cauchy, P, Hoogenkamp, M, Westhead, D R, Lacaud, G, Kouskoff, V, Göttgens, B & Bonifer, C 2016, ' Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation ', Developmental cell, vol. 36, no. 5, pp. 572-587 . https://doi.org/10.1016/j.devcel.2016.01.024 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2016.01.024 |
| Popis: | Summary Metazoan development involves the successive activation and silencing of specific gene expression programs and is driven by tissue-specific transcription factors programming the chromatin landscape. To understand how this process executes an entire developmental pathway, we generated global gene expression, chromatin accessibility, histone modification, and transcription factor binding data from purified embryonic stem cell-derived cells representing six sequential stages of hematopoietic specification and differentiation. Our data reveal the nature of regulatory elements driving differential gene expression and inform how transcription factor binding impacts on promoter activity. We present a dynamic core regulatory network model for hematopoietic specification and demonstrate its utility for the design of reprogramming experiments. Functional studies motivated by our genome-wide data uncovered a stage-specific role for TEAD/YAP factors in mammalian hematopoietic specification. Our study presents a powerful resource for studying hematopoiesis and demonstrates how such data advance our understanding of mammalian development. Graphical Abstract Highlights • Comprehensive genome-scale resource for studying embryonic blood cell specification • Genome-scale definition of cis elements driving differential gene expression • A gene regulatory network model for hematopoiesis aiding reprogramming experiments • Analysis suggests a role for TEAD factors in hematopoietic specification Goode, Obier, Vijayabaskar et al. isolate cells at six different stages of hematopoietic differentiation, starting from embryonic stem cells, and perform a comprehensive multi-omics analysis of this developmental pathway. The data identify regulators of hematopoietic specification and highlight the minimum requirements for the reprogramming of non-blood cells to blood. |
| Databáze: | OpenAIRE |
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