Cloning and Characterization of the Genes Encoding a Cytochrome P450 (PipA) Involved in Piperidine and Pyrrolidine Utilization and Its Regulatory Protein (PipR) in Mycobacterium smegmatis mc2155
| Autor: | Poupin, Pascal, Ducrocq, Véronique, Hallier-Soulier, Sylvie, Truffaut, Nicole |
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| Přispěvatelé: | Université de Technologie de Compiègne (UTC) |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Pyrrolidines
Molecular Sequence Data Mycobacterium smegmatis Sequence Homology Genetics and Molecular Biology Open Reading Frames Bacterial Proteins Cytochrome P-450 Enzyme System Piperidines [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Amino Acid Sequence Cloning Molecular ComputingMilieux_MISCELLANEOUS Base Sequence Escherichia coli Proteins Sequence Analysis DNA DNA-Binding Proteins Repressor Proteins Blotting Southern Kinetics Mutagenesis Insertional [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Glucose Genes Bacterial DNA Transposable Elements [SDE.BE]Environmental Sciences/Biodiversity and Ecology Transcription Factors |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 1999, 181 (11), pp.3419-3426. ⟨10.1128/JB.181.11.3419-3426.1999⟩ |
| ISSN: | 0021-9193 1098-5530 |
| DOI: | 10.1128/JB.181.11.3419-3426.1999⟩ |
| Popis: | Transposon mutagenesis of Mycobacterium smegmatis mc2155 enabled the isolation of a mutant strain (called LGM1) altered in the regulation of piperidine and pyrrolidine utilization. The complete nucleotide sequence of the gene inactivated in mutant LGM1 was determined from the wild-type strain. This gene (pipR) encoded a member of the GntR family of bacterial regulatory proteins. An insertion element (IS1096), previously described for M. smegmatis, was detected downstream of the gene pipR. Three additional open reading frames were found downstream of IS1096. The first open reading frame (pipA) appeared to encode a protein identified as a cytochrome P450 enzyme. This gene is the first member of a new family, CYP151. By a gene replacement experiment, it was demonstrated that the cytochrome P450 pipA gene is required for piperidine and pyrrolidine utilization in M. smegmatis mc2155. Genes homologous to pipA were detected by hybridization in several, previously isolated, morpholine-degrading mycobacterial strains. A gene encoding a putative [3Fe-4S] ferredoxin (orf1) and a truncated gene encoding a putative glutamine synthetase (orf2') were found downstream of pipA. |
| Databáze: | OpenAIRE |
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