TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
| Autor: | Li, Z., Li, B., Shen, W.H., Huang, H., Dong, A. |
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| Přispěvatelé: | School of Life Sciences [Fudan University], Fudan University [Shanghai], Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Chinese Academy of Sciences [Beijing] (CAS), The work was partly supported by grants from the National Basic Research Program of China (973 Program) 2012CB910500 and the Chinese National Scientific Foundation (31071064) to HH, and National Basic Research Program of China (973 Program) 2011CB944600 to AD., We thank Z. Liu and Y. Zhang for help with yeast two‐hybrid analysis and plasmid construction, respectively, P. Genschik for the Arabidopsis seedling cDNA library, S. Hake, M. Matuai and the Arabidopsis Biological Resource Center (ABRC) for Arabidopsis seeds, L. Xu for useful discussion of the manuscript and Abmart for the production of antibodies. |
| Rok vydání: | 2012 |
| Předmět: |
Homeodomain Proteins
MESH: RNA Plant/genetics MESH: Transcription Factors/metabolism MESH: Homeodomain Proteins/genetics Arabidopsis Proteins Arabidopsis food and beverages [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Arabidopsis/metabolism MicroRNAs Gene Expression Regulation Plant RNA Plant MESH: Arabidopsis Proteins/genetics MESH: Gene Expression Regulation Plant MESH: Homeodomain Proteins/metabolism MESH: Promoter Regions Genetic MESH: Arabidopsis/genetics MESH: MicroRNAs/genetics MESH: Arabidopsis/growth & development MESH: Transcription Factors/genetics MESH: Arabidopsis Proteins/metabolism Promoter Regions Genetic Transcription Factors |
| Zdroj: | Plant Journal Plant Journal, Wiley, 2012, 71, pp.99-107. ⟨10.1111/j.1365-313X.2012.04973.x⟩ |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1111/j.1365-313X.2012.04973.x⟩ |
| Popis: | International audience; Leaf organogenesis occurs within the peripheral zone of the shoot apical meristem (SAM). The initiation and subsequent development of a leaf requires the stable repression of a highly conserved class of plant genes, namely class-I KNOTTED 1-like homeobox (KNOX) genes. In Arabidopsis, this class comprises four members: SHOOT MERISTEMLESS (STM); BREVIPEDICELLUS (BP); KNAT2 and KNAT6. Two transcription factors, ASYMMETRIC LEAVES 1 (AS1) and AS2, are known to form a protein complex to repress BP, KNAT2 and KNAT6. Here, we show that AS2 physically interacts with the microRNA319 (miR319)-regulated CINCINNATA-like TEOSINTE BRANCHED 1-CYCLOIDEA-PCF (TCP) transcription factors in vitro and in vivo. By chromatin immunoprecipitation, we demonstrated that AS2 and TCPs bind to similar regions of the BP and KNAT2 promoters. In addition, DNA-binding activities of the TCP proteins rely on the presence of AS2, as the activities were dramatically reduced in the as2 mutant. The jaw-D mutant, which overexpresses MIR319a to downregulate several target TCP genes, strongly enhanced the as2 phenotypes and caused more severe ectopic expression of BP, KNAT2 and KNAT6. Our results reveal that KNOX repression requires different types of transcription factors that function together to ensure normal leaf development. |
| Databáze: | OpenAIRE |
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