| Autor: |
Tie-Ying, Qiu, Jin, Huang, Li-Ping, Wang, Bi-Song, Zhu |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Cell Journal (Yakhteh) |
| ISSN: |
2228-5806 |
| Popis: |
Objective Patients with diabetes mellitus frequently have chronic wounds or diabetic ulcers as a result of impaired wound healing, which may lead to limb amputation. Human umbilical vein endothelial cell (HUVEC) dysfunction also delays wound healing. Here, we investigated the mechanism of miR-200b in HUVECs under high glucose conditions and the potential of miR-200b as a therapeutic target. Materials and Methods In this experimental study, HUVECs were cultured with 5 or 30 mM glucose for 48 hours. Cell proliferation was evaluated by CCK-8 assays. Cell mobility was tested by wound healing and Transwell assays. Angiogenesis was analyzed in vitro Matrigel tube formation assays. Luciferase reporter assays were used to test the binding of miR-200b with Notch1. Results miR-200b expression was induced by high glucose treatment of HUVECs (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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