[Effect of tumor necrosis factor DNA on immune response to DNA immunization against herpes simplex virus]
| Autor: | Rr, Klimova, Kozlov AIu, Ln, Shingarova, Oksana Nekrasova, Ef, Boldyreva, Ts, Guseva, Ov, Parshina, Vv, Malinovskaia, Vv, Novikov, Aa, Kushch |
|---|---|
| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | ResearcherID Europe PubMed Central |
| ISSN: | 0026-8984 |
| Popis: | A study was made of the adjuvant effect of the mouse tumor necrosis factor alpha (mTNF alpha) on DNA immunization against the herpes simplex virus type 1 (HSV1). The HSV1 gD gene (pDNAgD) served as an immunogen; mTNF alpha or its gene cloned in an eukaryotic expression vector (pDNAmTNF) were used to modulate the immune response. Double immunization with pDNAgD led to a sixfold increase in the in vitro T-cell response, a high (1:2000) titer of anti-HSV1 antibodies (including virus-neutralizing antibodies), an increase in IgG2a/IgG1 (suggesting a shift of the immune response to the Th1 type), and no change in CD4/CD8 T-cell ratio. A single injection of mTNF alpha along with inactivated HSV1 allowed a twice higher antibody titer and a fourfold higher T-cell response as compared with immunization with HSV1 alone. Double immunization with both pDNAgD and pDNAmTNF increased the titer of anti-HSV1 antibodies and the T-cell response by factors of 8 and 1.5, respectively, as compared with immunization with pDNAgD alone. However, the protective effect was significantly lower with the two plasmids than with pDNAgD (73 vs. 100%). Thus, DNA immunization with pDNAgD induced both B- and T-cell responses and completely protected mice from a lethal doze of HSV1. The adjuvant properties of mTNF alpha and pDNAmTNF need further investigation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|