An adenovirus carrying the rat protein tyrosine phosphatase eta suppresses the growth of human thyroid carcinoma cell lines in vitro and in vivo
| Autor: | Iuliano, R., Trapasso, F., Le Pera, I., filippo schepis, Sama, I., Clodomiro, A., Dumon, K. R., Santoro, M., Chiariotti, L., Viglietto, G., Fusco, A. |
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| Přispěvatelé: | Iuliano, R, Trapasso, F, LE PERA, I, Schepis, F, Sama, I, Clodomiro, A, Dumon, Kr, Santoro, Massimo, Chiariotti, Lorenzo, Viglietto, G, Fusco, Alfredo |
| Rok vydání: | 2003 |
| Předmět: |
EXPRESSION
PHOSPHOLIPASE C-GAMMA Genetic Vectors Mice Nude Cell Cycle Proteins THERAPY Adenoviridae Mice Adenocarcinoma Follicular Animals Cell Division Cyclin-Dependent Kinase Inhibitor p27 Gene Therapy Humans Isoenzymes Nude Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases Phospholipase C gamma Phosphorylation Protein Tyrosine Phosphatases Rats Thyroid Neoplasms Transduction Genetic Tumor Cells Cultured Tumor Suppressor Proteins Type C Phospholipases Xenograft Model Antitumor Assays Transduction Genetic Adenocarcinoma Follicular Tumor Cells Cultured DEP-1 Genetic Therapy |
| Zdroj: | Cancer research (Chic. Ill.) 63 (2003): 882–886. info:cnr-pdr/source/autori:Iuliano R. 1, Trapasso F. 1, Le Pera I. 1, Schepis F. 1, Sama I. 1, Clodomiro A. 1, Dumon K.R. 2, Santoro M. 3, Chiariotti L. 1, Viglietto G. 1, Fusco A. 1-3/titolo:An adenovirus carrying the rat protein tyrosine phosphatase eta suppresses the growth of human thyroid carcinoma cell lines in vitro and in vivo./doi:/rivista:Cancer research (Chic. Ill.)/anno:2003/pagina_da:882/pagina_a:886/intervallo_pagine:882–886/volume:63 ResearcherID |
| Popis: | We demonstrated previously that rat tyrosine phosphatase r-PTPeta expression was suppressed in rat and human thyroid neoplastic cells, and that restoration of r-PTPeta expression reverted the malignant phenotype. To investigate the potential of this gene for cancer therapy, we generated an adenovirus carrying the r-PTPeta cDNA (Ad-r-PTPeta). This virus infected human thyroid carcinoma cells and overexpressed the r-PTPeta protein. Overexpression of r-PTPeta significantly inhibited the growth of four thyroid carcinoma cell lines. Cell growth inhibition was associated with down-regulation of extracellular signal-regulated kinase 1/2 activity, with increased levels of the cell-cycle inhibitor p27(kip1) protein and with dephosphorylation of PLCgamma1, a substrate of DEP-1, the human homologue of r-PTPeta. Finally, the growth of xenograft tumors induced in athymic mice by anaplastic thyroid carcinoma ARO cells transduced with the Ad-r-PTPeta virus was drastically reduced. These data suggest that gene therapy based on restoration of PTPeta function has potential in the treatment of human thyroid malignant neoplasias. |
| Databáze: | OpenAIRE |
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