Mitochondria mediate septin cage assembly to promote autophagy of Shigella
| Autor: | Sirianni A, Krokowski S, Lobato-Márquez D, Buranyi S, Pfanzelter J, Galea D, Willis A, Culley S, Ricardo Henriques, Larrouy-Maumus G, Hollinshead M, Sancho-Shimizu V, Way M, Mostowy S |
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| Přispěvatelé: | Wellcome Trust |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Biochemistry & Molecular Biology
autophagy Cell Cycle Proteins macromolecular substances 0601 Biochemistry and Cell Biology Models Biological Mitochondrial Dynamics Article ACTIN Cell Line Mitochondrial Proteins HOST-DEFENSE Humans septin PROTEIN DRP1 Science & Technology fungi RECOGNITION cytoskeleton Cell Biology Articles FISSION Microbiology Virology & Host Pathogen Interaction Mitochondria Cytoskeletal Proteins MAMMALIAN SEPTINS Autophagy & Cell Death Shigella MEMBRANE biological phenomena cell phenomena and immunity Life Sciences & Biomedicine STARVATION Septins LISTERIA Protein Binding Developmental Biology |
| Zdroj: | EMBO Reports CIÊNCIAVITAE Europe PubMed Central |
| ISSN: | 1469-3178 1469-221X |
| Popis: | Septins, cytoskeletal proteins with well‐characterised roles in cytokinesis, form cage‐like structures around cytosolic Shigella flexneri and promote their targeting to autophagosomes. However, the processes underlying septin cage assembly, and whether they influence S. flexneri proliferation, remain to be established. Using single‐cell analysis, we show that the septin cages inhibit S. flexneri proliferation. To study mechanisms of septin cage assembly, we used proteomics and found mitochondrial proteins associate with septins in S. flexneri‐infected cells. Strikingly, mitochondria associated with S. flexneri promote septin assembly into cages that entrap bacteria for autophagy. We demonstrate that the cytosolic GTPase dynamin‐related protein 1 (Drp1) interacts with septins to enhance mitochondrial fission. To avoid autophagy, actin‐polymerising Shigella fragment mitochondria to escape from septin caging. Our results demonstrate a role for mitochondria in anti‐Shigella autophagy and uncover a fundamental link between septin assembly and mitochondria. |
| Databáze: | OpenAIRE |
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