Ly-6C is a marker of memory CD8+ T cells
| Autor: | Tl, Walunas, Ds, Bruce, Lynn Dustin, Dy, Loh, Ja, Bluestone |
|---|---|
| Předmět: |
Male
Mice Inbred C3H CD3 Complex Receptors Antigen T-Cell Receptors Lymphocyte Homing Antibodies Monoclonal Mice Transgenic Receptors Cell Surface CD8-Positive T-Lymphocytes Mice Inbred C57BL Enterotoxins Mice Hyaluronan Receptors Animals Antigens Ly Leukocyte Common Antigens Carrier Proteins Immunologic Memory |
| Zdroj: | Europe PubMed Central |
| Popis: | This study examined long-term phenotypic and functional effects of TCR ligation in vivo. Flow cytometric analysis of T cells from mice treated with anti-CD3 revealed an increase in CD44 expression in both the CD4+ and CD8+ populations. The phenotypic changes were a result of TCR engagement, because treatment with staphylococcal enterotoxin B (SEB) resulted in a preferential increase in CD44 expression on the SEB-reactive V beta 8 T cells. In addition, the percentage of cells expressing Ly-6C increased among the CD8+ subset after anti-CD3 treatment and in the V beta 8+ CD8+ subset after treatment with SEB. Finally, the TCR transgenic (Tg) mouse strain 2C was used to confirm that the phenotypic changes can be induced by exposure to a physiologic ligand (H-2Ld). Before treatment, nearly all of the Tg+CD8+ cells were CD44low/Ly-6C-. Tg+ peritoneal exudate T cells isolated from mice challenged with P815 cells (H-2Ld) up-regulated Ly-6C and secreted higher levels of IFN-gamma on a per Tg+ CD8+ T cell basis after treatment. Taken together, these data indicate that in vivo TCR/CD3 engagement results in phenotypic and functional changes in T cells. Furthermore, Ly-6C expression correlates with an increase in IFN-gamma production after antigenic stimulation of CD8+ T cells, suggesting that it is a "memory" marker that correlates with Ag-specific functional changes in CD8+ T cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|