Structure and Immunogenicity of a Peptide Vaccine Including the Complete HIV-1 gp41 2F5 Epitope. Implications for Antibody Recognition Mechanism and Immunogen Design
| Autor: | Serrano S, Araujo A, Apellániz B, Bryson S, Carravilla P, De la Arada I, Huarte N, Rujas E, Emil Pai, Jl, Arrondo, Domene C, Ma, Jiménez, Jl, Nieva |
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NMR STRUCTURE
Protein Folding Spectrophotometry Infrared MEDIATED FUSION Nuclear Magnetic Resonance viruses Phosphorylcholine Immunology Molecular Sequence Data Fourier Transform IR (FTIR) HIV Antibodies Antibodies Viral Protein Structure Secondary Vaccine Development PROXIMAL EXTERNAL REGION Humans Amino Acid Sequence Micelles AIDS Vaccines Immunodominant Epitopes virus diseases Antibodies Monoclonal Peptide Conformation IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN Antibodies Neutralizing HIV Envelope Protein gp41 Protein Structure Tertiary MOLECULAR-DYNAMICS Liposomes Vaccines Subunit HIV-1 FORCE-FIELD MEMBRANE HUMAN MONOCLONAL-ANTIBODY BROADLY NEUTRALIZING ANTIBODIES Broadly Neutralizing Antibodies |
| Zdroj: | Europe PubMed Central Serrano, S, Araujo, A, Apellaniz, B, Bryson, S, Carravilla, P, de la Arada, I, Huarte, N, Rujas, E, Pai, E F, Arrondo, J L R, Domene, C, Angeles Jimenez, M & Nieva, J L 2014, ' Structure and Immunogenicity of a Peptide Vaccine, Including the Complete HIV-1 gp41 2F5 Epitope : IMPLICATIONS FOR ANTIBODY RECOGNITION MECHANISM AND IMMUNOGEN DESIGN ', Journal of Biological Chemistry, vol. 289, no. 10, pp. 6565-6580 . https://doi.org/10.1074/jbc.M113.527747 Digital.CSIC. Repositorio Institucional del CSIC instname |
| DOI: | 10.1074/jbc.M113.527747 |
| Popis: | Background: HIV-1 vaccines should elicit broadly neutralizing antibodies as the gp41 >membrane-proximal external region> targeting MAb2F5. Results: NMR disclosed unprecedented 2F5 peptide-epitope structures. Although overall conformation was preserved in different adjuvants, recovered antibodies after vaccination were functionally different. Conclusion: Membrane-inserted helical oligomers may encompass effective 2F5 peptide vaccines. Significance: Disclosing the structures that generate 2F5-like antibodies may guide future vaccine development. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. |
| Databáze: | OpenAIRE |
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