Phenotypic characterization of porcine interfollicular keratinocytes separated by elutriation: A technical note

Autor:	Hj, Gabius, Motlík J, Klima J, Karel Smetana, Jr.
Předmět:	Keratinocytes Binding Sites Phenotype Swine Animals Keratins Cell Separation Cell Fractionation
Zdroj:	Scopus-Elsevier Publons
Popis:	Separation of epidermal stem cells from other populations in suspensions of epidermal cells by sorting is hampered by a present lack of specific surface markers of this cell type. To address this issue we applied CCE combined with immunocytochemical phenotyping. On the basis of expression profiles for keratins (10, 14, and 19), nucleostemin, galectin-1 and epitopes reactive for this adhesion/growth-regulatory tissue lectin we identified a fraction of very small cells originating from the basal layer. The results demonstrated that CCE has potential merit for separation of epidermal cells to yield a population likely enriched in stem cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::99784518fcd4c07969ea85302c45e472 http://www.scopus.com/inward/record.url?eid=2-s2.0-33847374312&partnerID=MN8TOARS Zobrazit plný text záznamu