High NRBP1 expression in prostate cancer is linked with poor clinical outcomes and increased cancer cell growth
| Autor: | Christian, Ruiz, Martin, Oeggerli, Markus, Germann, Silvia, Gluderer, Hugo, Stocker, Mariacarla, Andreozzi, George N, Thalmann, Marco G, Cecchini, Tobias, Zellweger, Susanna, Stürm, Pasi A, Koivisto, Heikki J, Helin, Edward P, Gelmann, Andrew G, Glass, Thomas C, Gasser, Luigi M, Terracciano, Alexander, Bachmann, Stephen, Wyler, Lukas, Bubendorf, Cyrill A, Rentsch |
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| Rok vydání: | 2012 |
| Předmět: |
Aged
80 and over Male Prostatectomy Prostatic Hyperplasia Vesicular Transport Proteins Gene Expression Prostatic Neoplasms Receptors Cytoplasmic and Nuclear Adenocarcinoma Middle Aged Prognosis Survival Rate Ki-67 Antigen Tissue Array Analysis Biomarkers Tumor Humans RNA Interference RNA Small Interfering Finland Switzerland Aged |
| Zdroj: | The Prostate |
| DOI: | 10.1002/pros.22521 |
| Popis: | BACKGROUND We recently established the rationale that NRBP1 (nuclear receptor binding protein 1) has a potential growth promoting role in cell biology. NRBP1 interacts directly with TSC 22 a potential tumor suppressor gene that is differently expressed in prostate cancer. Consequently we analyzed the role of NRBP1 expression in prostate cancer cell lines and its expression on prostate cancer tissue microarrays (TMA). METHODS The effect of NRBP1 expression on tumor cell growth was analyzed by using RNAi. NRBP1 protein expression was evaluated on two TMAs containing prostate samples from more than 1000 patients. Associations with clinico pathological features the proliferation marker Ki67 and survival data were analyzed. RESULTS RNAi mediated silencing of NRBP1 expression in prostate cancer cell lines resulted in reduced cell growth (P?0.05). TMA analysis revealed NRBP1 protein expression in benign prostate hyperplasia in 6 as compared to 60 in both high grade intraepithelial neoplasia and prostate cancer samples. Strong NRBP1 protein expression was restricted to prostate cancer and correlated with higher expression of the proliferation marker Ki67 (P? |
| Databáze: | OpenAIRE |
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