The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes
| Autor: | Olombrada, Miriam, Peña, Cohue, Rodríguez-Galán, Olga, Klingauf-Nerurkar, Purnima, Portugal-Calisto, Daniela, Oborská-Oplová, Michaela, Altvater, Martin, Gavilanes, José G, Martínez-del-Pozo, Álvaro, de la Cruz, Jesús, García-Ortega, Lucía, Panse, Vikram Govind |
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| Přispěvatelé: | University of Zurich, Swiss National Science Foundation, National Centres of Competence in Research (Switzerland), ETH Zurich, European Research Council, Olga Mayenfisch Foundation, European Commission, Ministerio de Economía y Competitividad (España), EMBO |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Bioquímica
Cell Nucleus Biología molecular AcademicSubjects/SCI00010 10179 Institute of Medical Microbiology Active Transport Cell Nucleus 610 Medicine & health Ricin Saccharomyces cerevisiae Ribosome Subunits Large Eukaryotic Fungal Proteins 1311 Genetics RNA Ribosomal Protein Biosynthesis Endoribonucleases RNA and RNA-protein complexes 570 Life sciences biology Cell Nucleolus |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname E-Prints Complutense. Archivo Institucional de la UCM E-Prints Complutense: Archivo Institucional de la UCM Universidad Complutense de Madrid Nucleic Acids Research Nucleic Acids Research, 48 (11) |
| DOI: | 10.5167/uzh-190530 |
| Popis: | The ribotoxin α-sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether α-sarcin targets the SRL only in mature 60S subunits remains unresolved. Here, we show that, in yeast, α-sarcin can cleave SRLs within late 60S pre-ribosomes containing mature 25S rRNA but not nucleolar/nuclear 60S pre-ribosomes containing 27S pre-rRNA in vivo. Conditional expression of α-sarcin is lethal, but does not impede early pre-rRNA processing, nuclear export and the cytoplasmic maturation of 60S pre-ribosomes. Thus, SRL-cleaved containing late 60S pre-ribosomes seem to escape cytoplasmic proofreading steps. Polysome analyses revealed that SRL-cleaved 60S ribosomal subunits form 80S initiation complexes, but fail to progress to the step of translation elongation. We suggest that the functional integrity of a α-sarcin cleaved SRL might be assessed only during translation. V.G.P. is supported by grants from the Swiss National Science Foundation; NCCR RNA & Disease; ETH Zurich, a Starting Grant Award [EURIBIO260676] from the European Research Council and the Olga Mayenfisch Stiftung; Spanish Ministry of Economy and Competitiveness (MINECO) and the European Union ERFD program [BFU2012-32404] to A.M.P., [BFU2016-75352-P] to J.d.l.C.; M.O. was recipient of an FPU predoctoral contract from the Spanish Ministry of Education and an EMBO short-stay fellowship. Funding for open access charge: Swiss National Science Foundation. |
| Databáze: | OpenAIRE |
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