Genetic programming of macrophages generates an in vitro model for the human erythroid island niche
| Autor: | Lopez-Yrigoyen, Martha, Yang, Cheng-Tao, Fidanza, Antonella, Cassetta, Luca, Taylor, A. Helen, McCahill, Angela, Sellink, Erica, von Lindern, Marieke, van den Akker, Emile, Mountford, Joanne C., Pollard, Jeffrey W., Forrester, Lesley M. |
|---|---|
| Přispěvatelé: | Academic Medical Center, Landsteiner Laboratory, AII - Inflammatory diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Erythrocytes
Erythroblasts Macrophages Science Induced Pluripotent Stem Cells Kruppel-Like Transcription Factors Antigens CD34 Hematopoietic Stem Cells Interleukin-33 Article Angiopoietin-like Proteins Blood Substitutes Plasminogen Activator Inhibitor 2 Humans Blood Transfusion Erythropoiesis lcsh:Q Angiopoietin-Like Protein 7 lcsh:Science |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019) Lopez-Yrigoyen, M, Yang, C-T, Fidanza, A, Cassetta, L, Taylor, A H, McCahill, A, Sellink, E, von Lindern, M, van den Akker, E, Mountford, J C, Pollard, J W & Forrester, L M 2019, ' Genetic programming of macrophages generates an in vitro model for the human erythroid island niche ', Nature Communications, vol. 10, 881 . https://doi.org/10.1038/s41467-019-08705-0 Nature communications, 10(1):881. Nature Publishing Group Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-08705-0 |
| Popis: | Red blood cells mature within the erythroblastic island (EI) niche that consists of specialized macrophages surrounded by differentiating erythroblasts. Here we establish an in vitro system to model the human EI niche using macrophages that are derived from human induced pluripotent stem cells (iPSCs), and are also genetically programmed to an EI-like phenotype by inducible activation of the transcription factor, KLF1. These EI-like macrophages increase the production of mature, enucleated erythroid cells from umbilical cord blood derived CD34+ haematopoietic progenitor cells and iPSCs; this enhanced production is partially retained even when the contact between progenitor cells and macrophages is inhibited, suggesting that KLF1-induced secreted proteins may be involved in this enhancement. Lastly, we find that the addition of three secreted factors, ANGPTL7, IL-33 and SERPINB2, significantly enhances the production of mature enucleated red blood cells. Our study thus contributes to the ultimate goal of replacing blood transfusion with a manufactured product. In vitro differentiation of red blood cells (RBCs) is a desirable therapy for various disorders. Here the authors develop a culture system using stem cell-derived macrophages to show that inducible expression of a transcription factor, KLF1, enhances RBC production, potentially through the induction of three soluble factors, ANGPTL7, IL33 and SERPINB2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|