Establishment of a novel collagenase perfusion method to isolate rat pancreatic stellate cells and investigation of their gene expression of TGF-beta1, type I collagen, and CTGF in primary culture or freshly isolated cells
| Autor: | Toshiyuki, Shinji, Kozo, Ujike, Koji, Ochi, Nobuchika, Kusano, Tetsuya, Kikui, Naoki, Matsumura, Yasuyuki, Emori, Toshinobu, Seno, Norio, Koide |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Male
transforming growth factor beta Gene Expression pancreatic stellate cell Rats Inbred Strains Cell Separation Fibrosis Collagen Type I Immediate-Early Proteins Rats WBN/Kob rat Perfusion Transforming Growth Factor beta1 Immunologic Techniques connective tissue growth factor Animals Intercellular Signaling Peptides and Proteins Microscopy Phase-Contrast Collagenases Rats Wistar Pancreas Cells Cultured collagenase perfusion |
| Zdroj: | Acta Medica Okayama. 56(4):211-218 |
| ISSN: | 0386-300X |
| Popis: | In studies of the pathogenesis of pancreatic fibrosis, pancreatic stellate cells (PSCs) have recently gained attention. In the present study, we established a new collagenase perfusion method through thoracic aorta cannulation to isolate PSCs, and we studied gene expression of TGF-beta1, type I collagen, and connective tissue growth factor using primary cultured PSCs. Our method facilitated PSC isolation, and by our new method, 4.3 +/- 1.2 x 10(6) PSCs were obtained from a rat. In comparing the expression of these genes with that of hepatic stellate cells (HSCs), we observed a similar pattern, although PSCs expressed type I collagen gene earlier than did HSCs. These results suggest that PSCs may play an important role in fibrosis of the pancreas, as HSCs do in liver fibrosis; in addition, PSCs may exist in a preactivated state or may be more easily activated than are HSCs. We also isolated the PSCs from a WBN/Kob rat, the spontaneous pancreatitis rat, and compared the gene expression with that from a normal rat. |
| Databáze: | OpenAIRE |
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