GD2 CAR T cells against human glioblastoma
| Autor: | Prapa, M., Chiavelli, C., Golinelli, G., Grisendi, G., Bestagno, M., Di Tinco, R., Dall'Ora, M., Neri, G., Candini, O., Spano, C., Petrachi, T., Bertoni, L., Carnevale, G., Pugliese, G., Depenni, R., Feletti, A., Iaccarino, C., Pavesi, G., Dominici, M. |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | npj Precision Oncology, Vol 5, Iss 1, Pp 1-14 (2021) NPJ Precision Oncology |
| Popis: | Glioblastoma is the most malignant primary brain tumor and is still in need of effective medical treatment. We isolated patient-derived glioblastoma cells showing high GD2 antigen expression representing a potential target for CAR T strategy. Data highlighted a robust GD2 CAR antitumor potential in 2D and 3D glioblastoma models associated with a significant and CAR T-restricted increase of selected cytokines. Interestingly, immunosuppressant TGF β1, expressed in all co-cultures, did not influence antitumor activity. The orthotopic NOD/SCID models using primary glioblastoma cells reproduced human histopathological features. Considering still-conflicting data on the delivery route for targeting brain tumors, we compared intracerebral versus intravenous CAR T injections. We report that the intracerebral route significantly increased the length of survival time in a dose-dependent manner, without any side effects. Collectively, the proposed anti-GD2 CAR can counteract human glioblastoma potentially opening a new therapeutic option for a still incurable cancer. |
| Databáze: | OpenAIRE |
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