Distinct proteomic profiles characterise non-erosive from erosive reflux disease
| Autor: | C, Calabrese, V, Marzano, A, Urbani, G, Lazzarini, M C, Valerii, G, Liguori, S, Di Molfetta, F, Rizzello, P, Gionchetti, M, Campieri, E, Spisni |
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| Přispěvatelé: | Calabrese C, Marzano V, Urbani A, Lazzarini G, Valerii MC, Liguori G, Di Molfetta S, Rizzello F, Gionchetti P, Campieri M, Spisni E. |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Proteomics Esophageal pH Monitoring Proteome NERD Biopsy Gene Expression Profiling Blotting Western ERD ESOPHAGEAL CELL PROLIFERATION Middle Aged Severity of Illness Index Endoscopy Gastrointestinal Young Adult Case-Control Studies Gastroesophageal Reflux Humans Female SHOTGUN PROTEOMICS ANALYSIS Biomarkers Aged |
| Popis: | BACKGROUND: Erosive reflux disease (ERD) and non-erosive reflux disease (NERD) are often regarded as part of the spectrum of the same disease. AIM: To elucidate molecular features that characterise NERD and ERD at the protein level. METHODS: A total of 56 consecutive subjects were enrolled: 10 healthy subjects, 24 with NERD and 22 with ERD. Eight specimens were taken from macroscopically normal mucosa at 5 cm of gastro-oesophageal junction. Four were processed for the proteins extraction and four for evaluation using haematoxylin-eosin and immunohistochemistry. We used shotgun proteomics to identify tentative disease molecular features for ERD or NERD. Candidate distinctive proteins were verified using immunohistochemistry. RESULTS: Shotgun proteomics analysis revealed 33 differentially expressed proteins in NERD vs. ERD samples, involved in cellular proliferation, keratinisation, stress responses and sugar metabolism. Based on a gene ontology meta-analysis, seven of them were further analysed using Western blotting (WB) and four also using immunohistochemistry. We identified novel candidate disease molecular features for GERD and few distinctive proteins to discriminate NERD and ERD. In particular, Transitional Endoplasmic Reticulum ATPase (TER ATPase), GAPDH, Alpha 1 Acid Glycoprotein 1, Annexin A1, Calmodulin and 14-3-3 proteins were confirmed at WB analysis. CONCLUSIONS: Non-erosive reflux disease and ERD are distinct disease entities at the protein level. This study proposes an array of candidate biomarkers possibly useful to discriminate between NERD and ERD. |
| Databáze: | OpenAIRE |
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