Mammary gland transcriptome and proteome modifications by nutrient restriction in early lactation Holstein cows challenged with intramammary lipopolysaccharide

Autor: Karol Pawłowski, José A.A. Pires, Yannick Faulconnier, Christophe Chambon, Pierre Germon, Céline Boby, Christine Leroux
Přispěvatelé: Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), American Society of Animal Science (ASAS). USA., American Dairy Science Association (ADSA). USA., Canadian Society of Animal Science (CSAS). CAN., Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), GISA meta-program of INRA (Ruminflame project), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-Université de Tours
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Lipopolysaccharides
Proteomics
mammary gland
[SDV]Life Sciences [q-bio]
approche protéomique
plateforme exploration du métabolisme Clermont-Ferrand
Article
[SHS]Humanities and Social Sciences
lcsh:Chemistry
cows
Mammary Glands
Animal
Nutrigenomics
[SDV.IDA]Life Sciences [q-bio]/Food engineering
Animals
Lactation
[INFO]Computer Science [cs]
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
analyse du transcriptome
lcsh:QH301-705.5
transcriptomic analysis
ComputingMilieux_MISCELLANEOUS
Caloric Restriction
Oligonucleotide Array Sequence Analysis
Gene Expression Profiling
Nutritional Requirements
race holstein
lipopolisaccharide
proteomic analysis
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
inflammation
mammary omics
undernutrition
inflammation
glande mammaire
restriction alimentaire
Cattle
Female
Zdroj: 2016 JAM Joint Annual Meeting
2016 JAM Joint Annual Meeting, Jul 2016, Salt LAke City, United States. American Society of Animal Science / American Dairy Science Association American Dairy Science Association, Journal of Animal Science, 94, 2016, 2016 JAM, Joint Annual Meeting, "Animals and Science: Big Solutions for Grand Challenges",July19-23, 2016, Salt Lake City, Utah, USA
International Journal of Molecular Sciences
International Journal of Molecular Sciences, MDPI, 2019, 20 (5), 19 p. ⟨10.3390/ijms20051156⟩
2016 JAM, Joint Annual Meeting, "Animals and Science: Big Solutions for Grand Challenges",July19-23, 2016, Salt Lake City, Utah, USA. (94)2016; 2016 JAM Joint Annual Meeting, Salt LAke City, USA, 2016-07-19-2016-07-23, 59-60
International Journal of Molecular Sciences, Vol 20, Iss 5, p 1156 (2019)
ISSN: 1661-6596
1422-0067
DOI: 10.3390/ijms20051156⟩
Popis: The objective is to study the effects of nutrient restrictions, which induce a metabolic imbalance on the inflammatory response of the mammary gland in early lactation cows. The aim is to decipher the molecular mechanisms involved, by comparing a control, with a restriction group, a transcriptome and proteome, after an intra-mammary lipopolysaccharide challenge. Multi-parous cows were either allowed ad libitum intake of a lactation diet (n = 8), or a ration containing low nutrient density (n = 8; 48% barley straw and dry matter basis) for four days starting at 24 ± 3 days in milk. Three days after the initiation of their treatments, one healthy rear mammary quarter of 12 lactating cows was challenged with 50 µg of lipopolysaccharide (LPS). Transcriptomic and proteomic analyses were performed on mammary biopsies obtained 24 h after the LPS challenge, using bovine 44K microarrays, and nano-LC-MS/MS, respectively. Restriction-induced deficits in energy, led to a marked negative energy balance (41 versus 97 ± 15% of Net Energy for Lactation (NEL) requirements) and metabolic imbalance. A microarray analyses identified 25 differentially expressed genes in response to restriction, suggesting that restriction had modified mammary metabolism, specifically β-oxidation process. Proteomic analyses identified 53 differentially expressed proteins, which suggests that the modification of protein synthesis from mRNA splicing to folding. Under-nutrition influenced mammary gland expression of the genes involved in metabolism, thereby increasing β-oxidation and altering protein synthesis, which may affect the response to inflammation.
Databáze: OpenAIRE
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