Epidemiology and genetic characterization of BVDV, BHV-1, BHV-4, BHV-5 and Brucella spp. infections in cattle in Turkey
| Autor: | ASLAN, Muhammet Eren, AZKUR, Ahmet Kursat, GAZYAGCI, Serkal |
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| Přispěvatelé: | Kırıkkale Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Turkey animal diseases viruses Brucellosis Bovine Meningoencephalitis Virology Animals Encephalitis Viral Herpesviridae Herpesvirus 1 Bovine Herpesvirus 5 Bovine Diarrhea Viruses Bovine Viral Full Paper seroprevalence phylogenetic analysis virus diseases Herpesviridae Infections biochemical phenomena metabolism and nutrition abortion agent Brucella Herpesvirus 4 Bovine Tumor Virus Infections cattle DNA Viral RNA Viral Bovine Virus Diarrhea-Mucosal Disease |
| Zdroj: | The Journal of Veterinary Medical Science |
| Popis: | GAZYAGCI, SERKAL/0000-0002-0043-6942 WOS: 000373536800005 PubMed: 26096964 The aim of the study was to determine the epidemiological data of bovine viral diarrhea virus (BVDV), bovine herpesvirus-1 (BHV-1), bovine herpesvirus-4 (BHV-4), bovine herpesvirus-5 (BHV-5) and Brucella associated cattle that were previously reported to have abortion and infertility problems in Ankara, Corum, Kirikkale and Yozgat provinces, Turkey. Whole blood and sera samples were obtained from 656 cattle, and antibodies against Brucella spp. were detected in 45 (6.86%) and 41 (6.25%) animals by Rose Bengal plate and serum tube agglutination tests, respectively. The seropositivity rates against BVDV, BHV-1 and BHV-4 were 70.89%, 41.3% and 28.78%, respectively. RT-PCR and PCR were performed to detect RNA and DNA viruses in blood samples, respectively. The BVDV 5'-untranslated region and BHV-1 gB gene detected in this study were phylogenetically analyzed. The BVDV strains analyzed in this study were closely related to those previously reported from Turkey. The nucleotide sequence from the BHV-1 strain detected in this study is the first nucleotide sequence of BHV-1 circulating in this area of Turkey deposited in the GenBank. The presence of Brucella spp. and prevalence of BHV-1, BHV-4 and BVDV in cattle should be further investigated throughout these regions. |
| Databáze: | OpenAIRE |
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