Combined use of an antigen and antibody detection enzyme-linked immunosorbent assay for cysticercosis as tools in an epidemiological study of epilepsy in Burundi

Autor: Prado-Jean, Annie, Kanobana, K., Druet-Cabanac, Michel, Nsengiyumva, Georges, Dorny, P., Preux, Pierre-Marie, Geerts, Stanny
Přispěvatelé: Neuroépidémiologie Tropicale et Comparée (NETEC), Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Université de Limoges (UNILIM), Service de Santé au Travail [CHU Limoges], CHU Limoges, Unité Fonctionnelle Registre Général des Cancers du Limousin (UFRGC), Centre National de Référence en matière de VIH SIDA, Université de Bujumbura, Laboratoire de Biostatistique et d'Informatique Médicale, Université de Limoges (UNILIM), Service de l'Information Médicale et de l'Évaluation [CHU Limoges] (SIME)
Rok vydání: 2007
Předmět:
Antigen detection
Adult
Male
Burundi
Antibodies
Helminth
MESH: DNA
Protozoan
Enzyme-Linked Immunosorbent Assay
Neurocysticercosis
MESH: Antigens
Helminth
MESH: Neurocysticercosis
MESH: Genotype
MESH: Brain
MESH: Antibodies
Helminth
MESH: Risk Factors
Risk Factors
Humans
MESH: Animals
Africa
Central
MESH: Antiprotozoal Agents
MESH: Chorioretinitis
Sex Distribution
MESH: Burundi
MESH: Humans
MESH: Middle Aged
Epilepsy
Cysticercosis
MESH: Toxoplasma
MESH: Sex Distribution
Laboratory techniques and procedures
MESH: Enzyme-Linked Immunosorbent Assay
MESH: Adult
Helminthic diseases
MESH: HIV Infections
MESH: Case-Control Studies
MESH: Male
MESH: Toxoplasmosis
Cerebral
Antibody detection
MESH: Epilepsy
Laboratory diagnosis
Antigens
Helminth
Case-Control Studies
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
ELISA
Female
MESH: Female
Zdroj: Tropical Medicine and International Health
Tropical Medicine and International Health, Wiley-Blackwell, 2007, 12 (7), pp.895-901. ⟨10.1111/j.1365-3156.2007.01860.x⟩
ISSN: 1360-2276
1365-3156
DOI: 10.1111/j.1365-3156.2007.01860.x⟩
Popis: The definitive version is available at www3.interscience.wiley.com
OBJECTIVE: To evaluate the benefits of the detection of both circulating antibodies (Ab) and antigens (Ag) for the diagnosis of cysticercosis in people with epilepsy. Neurocysticercosis is a cause of neurological diseases world-wide, especially epilepsy. The clinical symptoms of neurocysticercosis are non-specific and diagnosis is often difficult. METHODS: Serum samples were collected from subjects in a matched case-control study for epilepsy in the Kiremba area, Burundi, between March and April 2001 (epileptic cases=303; controls without epilepsy=606). The enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies (Ab-ELISA) and circulating Ag (Ag-ELISA). RESULTS: The Ab-ELISA revealed 58.7% positivity in epilepsy cases and 31.4% in healthy controls; and Ag-ELISA revealed 38.3% positivity in epilepsy cases and 20.0% in controls. The matched odds ratios were 3.6 (95% CI: 2.5-4.9) for Ab-ELISA, and 2.9 (95% CI: 2.1-4.3) for Ag-ELISA. CONCLUSION: Both Ag- and Ab-ELISA detected a significantly higher number of seropositives among people with epilepsy than among controls. The risk of epilepsy was high in cases with a positive Ag-ELISA, although less important than in cases with positivity for Ab-ELISA. Dead or degenerating cysticerci appear to be more frequently associated with epilepsy than living cysts. The high number of people with circulating Ag of Taenia solium suggests that the study area is a focus of active transmission of the parasite.
Databáze: OpenAIRE
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