Primary structure and chromosomal localization of human and mouse rod photoreceptor cGMP-gated cation channel
| Autor: | Steven Pittler, Ak, Lee, Altherr MR, Ta, Howard, Mf, Seldin, Rl, Hurwitz, Jj, Wasmuth, Baehr W |
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| Předmět: |
Mice
Inbred C3H Base Sequence Centromere Molecular Sequence Data Restriction Mapping Chromosome Mapping Cyclic Nucleotide-Gated Cation Channels Hybrid Cells Polymerase Chain Reaction Ion Channels Retina Muridae Mice Open Reading Frames Sequence Homology Nucleic Acid Animals Humans Photoreceptor Cells Amino Acid Sequence RNA Messenger Chromosomes Human Pair 4 Cloning Molecular Eye Proteins Poly A Gene Library |
| Zdroj: | Europe PubMed Central |
| Popis: | We have determined the primary structures of the human and mouse retinal rod cGMP-gated cation channel by analysis of cDNA clones and amplified DNA. The open reading frames predicted polypeptides of 690 and 683 residues exhibiting 88% sequence similarity. Sequence comparison indicated that the rod channels consist of a variable 90-residue N-terminal region, a short highly charged segment rich in lysine and glutamate, and a 540-residue C-terminal portion that is well conserved in three mammalian species. Significant sequence similarity (59%) of the visual cGMP-gated channel to the olfactory cAMP-gated channel established the existence of a family of cyclic nucleotide-gated ion channel genes. RNA blot analysis revealed transcripts of 3.2 kilobases (kb) in human, mouse, and dog, 3.2, 4.6, and 5.2 kb in bovine, and 3.6 kb in fish. The human channel gene was mapped by polymerase chain reaction of somatic cell hybrid DNAs to chromosome 4 (p14-q13) near the centromere. The mouse channel gene locus (Cncg) was mapped by interspecific backcross haplotype analysis 0.9 centimorgan proximal of the Kit locus on chromosome 5. |
| Databáze: | OpenAIRE |
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