Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster

Autor: Valanne, Susanna, Salminen, Tiina S., Järvelä-Stölting, Mirva, Vesala, Laura, Rämet, Mika
Přispěvatelé: Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology, Tampere University
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Life Cycles
Hemocytes
Biochemistry
Fats
White Blood Cells
Larvae
Glucose Metabolism
Animal Cells
Medicine and Health Sciences
Biology (General)
Immune Response
Drosophila Melanogaster
Eukaryota
Animal Models
Genomics
Lipids
hemocytes
Insects
Nucleic acids
Experimental Organism Systems
CR44404
Carbohydrate Metabolism
Drosophila
Cellular Types
Transcriptome Analysis
Research Article
Arthropoda
Biolääketieteet - Biomedicine
QH301-705.5
Immune Cells
Immunology
Research and Analysis Methods
Biokemia
solu- ja molekyylibiologia - Biochemistry
cell and molecular biology
Model Organisms
Genetiikka
kehitysbiologia
fysiologia - Genetics
developmental biology
physiology
Genetics
Animals
Non-coding RNA
Blood Cells
Biology and life sciences
fungi
Organisms
Computational Biology
Cell Biology
RC581-607
Genome Analysis
immunity
Invertebrates
Metabolism
fatbody
Animal Studies
Long non-coding RNAs
RNA
Immunologic diseases. Allergy
metabolism
Developmental Biology
Zdroj: PLoS Pathogens, Vol 15, Iss 1, p e1007504 (2019)
PLoS Pathogens
ISSN: 1553-7374
1553-7366
Popis: Non-coding RNAs have important roles in regulating physiology, including immunity. Here, we performed transcriptome profiling of immune-responsive genes in Drosophila melanogaster during a Gram-positive bacterial infection, concentrating on long non-coding RNA (lncRNA) genes. The gene most highly induced by a Micrococcus luteus infection was CR44404, named Induced by Infection (lincRNA-IBIN). lincRNA-IBIN is induced by both Gram-positive and Gram-negative bacteria in Drosophila adults and parasitoid wasp Leptopilina boulardi in Drosophila larvae, as well as by the activation of the Toll or the Imd pathway in unchallenged flies. We show that upon infection, lincRNA-IBIN is expressed in the fat body, in hemocytes and in the gut, and its expression is regulated by NF-κB signaling and the chromatin modeling brahma complex. In the fat body, overexpression of lincRNA-IBIN affected the expression of Toll pathway -mediated genes. Notably, overexpression of lincRNA-IBIN in unchallenged flies elevated sugar levels in the hemolymph by enhancing the expression of genes important for glucose retrieval. These data show that lncRNA genes play a role in Drosophila immunity and indicate that lincRNA-IBIN acts as a link between innate immune responses and metabolism.
Author summary Drosophila melanogaster is a powerful genetic model for studying the innate immune mechanisms conserved from flies to humans. With recent methodology, such as whole transcriptome analyses, novel non-protein coding genes in addition to protein coding genes are being increasingly identified. These long and short non-coding RNA genes are located between and within protein coding genes in the genome, and their functions are largely uncharacterized. In humans, such RNA genes have been shown to affect numerous physiological processes including immune responses. In Drosophila, very few non-coding RNA genes have so far been characterized in detail. In this study, we have identified and characterized an immune-inducible long non-coding RNA gene, lincRNA-IBIN. lincRNA-IBIN is induced by exposure to bacteria as well as the parasitoid wasp, Leptopilina boulardi, suggesting a general role in humoral and cellular innate immunity. Accordingly, forced expression of lincRNA-IBIN enhances the expression of genes involved in carbohydrate catabolism and elevates hemolymph glucose levels in Drosophila. These results indicate that lincRNA-IBIN acts as a link between immunity and metabolism in Drosophila. As research in Drosophila has often resulted in the identification of evolutionarily conserved mechanisms also in mammals, it remains to be studied whether long non-coding RNA genes regulate metabolism upon an infection also in humans.
Databáze: OpenAIRE
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