Evidence for a role of phosphatidylinositol 3-kinase in the regulation of glucose transport in Xenopus oocytes
| Autor: | Gwyn Gould, Tj, Jess, Gc, Andrews, Jj, Herbst, Rj, Plevin, Em, Gibbs |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Dose-Response Relationship
Drug Microinjections Molecular Sequence Data Biological Transport Deoxyglucose Androstadienes Enzyme Activation Phosphatidylinositol 3-Kinases Phosphotransferases (Alcohol Group Acceptor) Xenopus laevis Glucose Oocytes Animals Amino Acid Sequence Insulin-Like Growth Factor I Wortmannin Oligopeptides Protein Binding |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0021-9258 |
| Popis: | We have used two experimental approaches to examine the possible role of phosphatidylinositol 3-kinase (PI 3-kinase) in the regulation of glucose transport in oocytes isolated from Xenopus laevis. Incubation of oocytes with the PI 3-kinase inhibitor wortmannin inhibited insulin-like growth factor-1-stimulated deoxyglucose uptake. Half-maximal inhibition was observed at concentrations approximately 20 nM. Conversely, we also examined the effects of microinjection of synthetic peptides designed to interact with Src homology 2 domains of the regulatory subunit of PI 3-kinase on deoxyglucose transport in oocytes. We show that a bifunctional synthetic peptide containing two YMXM consensus sequences for binding to SH2 domains stimulated both PI 3-kinase activity and deoxyglucose transport when both tyrosine residues were phosphorylated. However, non-phosphorylated or bisphosphonotyrosine peptides with the identical amino acid sequence failed to stimulate transport or PI 3-kinase activity. Taken together, these data argue strongly for a role for PI 3-kinase in the regulation of glucose transport in oocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|