THE VOLTAGE-GATED SODIUM CHANNEL BETA4 SUBUNIT MAINTAINS EPITHELIAL PHENOTYPE IN MAMMARY CELLS
| Autor: | Doray, Adélaïde, Lemoine, Roxane, Severin, Marc, Chadet, Stéphanie, Lopez-Charcas, Osbaldo, Héraud, Audrey, Baron, Christophe, Besson, Pierre, Monteil, Arnaud, Pedersen, Stine Falsig, Roger, Sébastien |
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| Přispěvatelé: | EA4245 - Transplantation, Immunologie, Inflammation [Tours] (T2i), Université de Tours (UT), University of Copenhagen = Københavns Universitet (KU), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Université de Tours, University of Copenhagen = Københavns Universitet (UCPH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Monteil, Arnaud |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
[SDV]Life Sciences [q-bio] NaVβ4 Down-Regulation Article Cell Line Mesoderm Mammary Glands Animal Epithelial-to-Mesenchymal Transition Animals Humans Biology (General) beta Catenin Voltage-Gated Sodium Channel beta-4 Subunit Cell Polarity Epithelial Cells β-catenin [SDV] Life Sciences [q-bio] Protein Subunits Phenotype Proteolysis Female mammary cells Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation epithelial-to-mesenchymal transition mammary cells epithelial phenotype |
| Zdroj: | Cells Cells, MDPI, 2021 Volume 10 Issue 7 Cells, 2021, ⟨10.3390/cells10071624⟩ Cells, Vol 10, Iss 1624, p 1624 (2021) Doray, A, Lemoine, R, Severin, M, Chadet, S, Lopez-Charcas, O, Héraud, A, Baron, C, Besson, P, Monteil, A, Pedersen, S F & Roger, S 2021, ' The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells ', Cells, vol. 10, no. 7, 1624 . https://doi.org/10.3390/cells10071624 |
| ISSN: | 2073-4409 |
| Popis: | The SCN4B gene, coding for the NaVβ4 subunit of voltage-gated sodium channels, was recently found to be expressed in normal epithelial cells and down-regulated in several cancers. However, its function in normal epithelial cells has not been characterized. In this study, we demonstrated that reducing NaVβ4 expression in MCF10A non-cancer mammary epithelial cells generated important morphological changes observed both in two-dimensional cultures and in three-dimensional cysts. Most notably, the loss of NaVβ4 induced a complete loss of epithelial organisation in cysts and increased proteolytic activity towards the extracellular matrix. Loss of epithelial morphology was associated with an increased degradation of β-catenin, reduced E-cadherin expression and induction of mesenchymal markers N-cadherin, vimentin, and α-SMA expression. Overall, our results suggest that Navβ4 may participate in the maintenance of the epithelial phenotype in mammary cells and that its downregulation might be a determining step in early carcinogenesis. |
| Databáze: | OpenAIRE |
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