Transcription activation of a UV-inducible Clostridium perfringens bacteriocin gene by a novel σ factor

Autor: Dupuy, Bruno, Mani, Nagraj, Katayama, Seiichi, Sonenshein, Abraham
Přispěvatelé: Génétique Moléculaire Bactérienne, Institut Pasteur [Paris], Department of Molecular Biology and Microbiology [Tufts, Boston], Tufts University School of Medicine [Boston], Okayama University of Science, This work was supported by a research grant (GM042219 to A.L.S.) and a project grant (DK34928 to the Tufts University Gastroenterology Research and Secretory Processes Center) from the US Public Health Service, by a postdoctoral fellowship (to N.M.) from the Charles A. King Trust, by a Grant‐in‐Aid for Scientific Research (to S.K.) from the Ministry of Education, Culture, Sports, Science and Technology (Japan), and by funds from the Institut Pasteur (Paris) (to B.D.)., We thank B. Belitsky, C. Squires, and S. S. Dineen for helpful discussions and comments on the manuscript., Institut Pasteur [Paris] (IP)
Jazyk: angličtina
Rok vydání: 2005
Předmět:
Transcriptional Activation
MESH: DNA Primers
Clostridium perfringens
Ultraviolet Rays
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Sigma Factor
MESH: Escherichia coli/genetics
MESH: Base Sequence
MESH: Bacterial Proteins/genetics
Polymerase Chain Reaction
MESH: Clostridium perfringens/radiation effects
Bacterial Proteins
Bacteriocins
MESH: Plasmids
Escherichia coli
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Promoter Regions
Genetic
DNA Primers
MESH: DNA Damage
MESH: Gene Expression Regulation
Bacterial
MESH: Molecular Sequence Data
Base Sequence
MESH: Sigma Factor/genetics
MESH: Bacteriocins/genetics
MESH: Polymerase Chain Reaction
Gene Expression Regulation
Bacterial
MESH: Clostridium perfringens/genetics
MESH: Promoter Regions
Genetic/genetics
MESH: Transcriptional Activation
MESH: Ultraviolet Rays
DNA Damage
Plasmids
Zdroj: Molecular Microbiology
Molecular Microbiology, Wiley, 2005, 55 (4), pp.1196-1206. ⟨10.1111/j.1365-2958.2004.04456.x⟩
Molecular Microbiology, 2005, 55 (4), pp.1196-1206. ⟨10.1111/j.1365-2958.2004.04456.x⟩
ISSN: 0950-382X
1365-2958
DOI: 10.1111/j.1365-2958.2004.04456.x⟩
Popis: International audience; Expression of the plasmid-encoded Clostridium perfringens gene for bacteriocin BCN5 was shown to depend in vivo and in vitro on the activity of UviA protein. UviA, also plasmid-encoded, proved to be an RNA polymerase sigma factor and was also partly autoregulatory. The uviA gene has two promoters; one provided a UviA-independent, basal level of gene expression while the stronger, UviA-dependent promoter was only utilized after the cell experienced DNA damage. As a result, BCN5 synthesis is induced by treatment with UV light or mitomycin C. UviA is related to a special class of sigma factors found to date only in Clostridium species and responsible for activating transcription of toxin genes in Clostridium difficile, Clostridium tetani, and Clostridium botulinum.
Databáze: OpenAIRE
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