Identification of a Brucella spp. secreted effector specifically interacting with human small GTPase Rab2
| Autor: | De Barsy, Marie, Jamet, Alexandre, Filopon, Didier, Nicolas, Cécile, Laloux, Géraldine, Rual, Jean-François, Muller, Alexandre, Twizere, Jean-Claude, Nkengfac, Bernard, Vandenhaute, Jean, Hill, David, Salcedo, Suzana, Gorvel, Jean-Pierre, Letesson, Jean-Jacques, De Bolle, Xavier |
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| Přispěvatelé: | Université de Namur [Namur], MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Center for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute [Boston]-Department of Cancer Biology, Unité de Recherche en Biologie Moléculaire, Facultés Universitaires Notre Dame de la Paix (FUNDP) - Namur, Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Center for Cellular and Molecular Biology, Université de Liège - Gembloux, Communauté Française de Belgique (Action de Recherches Concertées 04/09-325 and 08/13-015), Fonds National pour la Recherche Scientifique (FRS-FNRS, FRFC Grants 2.4521.04 and 2.4541.08), Ellison Foundation (awarded to Marc Vidal), Institute Sponsored Research from the DFCI Strategic Initiative in support of CCSB, University of Namur (FUNDP), Unité de recherche en biologie des micro-organismes [Namur] (URBM), Université de Namur [Namur] (UNamur), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Virulence Factors
Brucella abortus MESH: Virulence MESH: Two-Hybrid System Techniques Cell Line Mice MESH: Phagosomes Bacterial Proteins Phagosomes Two-Hybrid System Techniques Protein Interaction Mapping Animals Humans MESH: Protein Binding MESH: Animals MESH: Bacterial Proteins MESH: Brucella abortus MESH: Mice MESH: Virulence Factors MESH: Humans Virulence Macrophages MESH: Protein Interaction Mapping MESH: Host-Pathogen Interactions MESH: Macrophages Epithelial Cells MESH: Cell Line rab2 GTP-Binding Protein MESH: Epithelial Cells MESH: Gene Deletion Host-Pathogen Interactions [SDV.IMM]Life Sciences [q-bio]/Immunology Gene Deletion MESH: rab2 GTP-Binding Protein Protein Binding |
| Zdroj: | Cellular Microbiology Cellular Microbiology, Wiley, 2011, 13 (7), pp.1044-58. ⟨10.1111/j.1462-5822.2011.01601.x⟩ Cellular Microbiology, 2011, 13 (7), pp.1044-58. ⟨10.1111/j.1462-5822.2011.01601.x⟩ |
| ISSN: | 1462-5814 1462-5822 |
| DOI: | 10.1111/j.1462-5822.2011.01601.x⟩ |
| Popis: | International audience; Bacteria of the Brucella genus are facultative intracellular class III pathogens. These bacteria are able to control the intracellular trafficking of their vacuole, presumably by the use of yet unknown translocated effectors. To identify such effectors, we used a high-throughput yeast two-hybrid screen to identify interactions between putative human phagosomal proteins and predicted Brucella spp. proteins. We identified a specific interaction between the human small GTPase Rab2 and a Brucella spp. protein named RicA. This interaction was confirmed by GST-pull-down with the GDP-bound form of Rab2. A TEM-β-lactamase-RicA fusion was translocated from Brucella abortus to RAW264.7 macrophages during infection. This translocation was not detectable in a strain deleted for the virB operon, coding for the type IV secretion system. However, RicA secretion in a bacteriological culture was still observed in a ΔvirB mutant. In HeLa cells, a ΔricA mutant recruits less GTP-locked myc-Rab2 on its Brucella-containing vacuoles, compared with the wild-type strain. We observed altered kinetics of intracellular trafficking and faster proliferation of the B. abortusΔricA mutant in HeLa cells, compared with the wild-type control. Altogether, the data reported here suggest RicA as the first reported effector with a proposed function for B. abortus. |
| Databáze: | OpenAIRE |
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