A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte
| Autor: | Penrad-Mobayed, M., Perrin, C., L'Hôte, D., Contremoulins, V., Lepesant, J. A., Boizet-Bonhoure, B., Poulat, F., Baudin, X., Veitia, R. A. |
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| Přispěvatelé: | Larose, Catherine, Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
endocrine system
animal structures Transcription Genetic Science Xenopus [SDV]Life Sciences [q-bio] [SDV.GEN] Life Sciences [q-bio]/Genetics Chromosomes Article Xenopus laevis Cytosol Pleurodeles Animals RNA Messenger RNA Processing Post-Transcriptional Cell Nucleus [SDV.GEN]Life Sciences [q-bio]/Genetics SOX9 Transcription Factor Biological Evolution [SDV] Life Sciences [q-bio] Ribonucleoproteins embryonic structures Oocytes Medicine Female |
| Zdroj: | Scientific Reports Scientific Reports, 2018, 8 (1), pp.7191. ⟨10.1038/s41598-018-25356-1⟩ Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.7191. ⟨10.1038/s41598-018-25356-1⟩ Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) |
| ISSN: | 2045-2322 |
| Popis: | International audience; Sox9 is a member of the gene family of SOX transcription factors, which is highly conserved among vertebrates. It is involved in different developmental processes including gonadogenesis. In all amniote species examined thus far, Sox9 is expressed in the Sertoli cells of the male gonad, suggesting an evolutionarily conserved role in testis development. However, in the anamniotes, fishes and amphibians, it is also expressed in the oocyte but the significance of such an expression remains to be elucidated. Here, we have investigated the nuclear localization of the SOX9 protein in the oocyte of three amphibian species, the urodelan Pleurodeles waltl, and two anurans, Xenopus laevis and Xenopus tropicalis. We demonstrate that SOX9 is associated with ribonucleoprotein (RNP) transcripts of lampbrush chromosomes in an RNA-dependent manner. This association can be visualized by Super-resolution Structured Illumination Microscopy (SIM). Our results suggest that SOX9, known to bind DNA, also carries an additional function in the posttranscriptional processes. We also discuss the significance of the acquisition or loss of Sox9 expression in the oocyte during evolution at the transition between anamniotes and amniotes. |
| Databáze: | OpenAIRE |
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