An evolutionarily-conserved Wnt3/β-catenin/Sp5 feedback loop restricts head organizer activity in Hydra
| Autor: | Matthias C. Vogg, Leonardo Beccari, Laura Iglesias Ollé, Christine Rampon, Sophie Vriz, Chrystelle Perruchoud, Yvan Wenger, Brigitte Galliot |
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| Rok vydání: | 2018 |
| Předmět: |
Evolution
Hydra Science Sp5 transcription factor Article Wnt3 Protein ddc:590 Head activator Animals Regeneration Cnidarians lcsh:Science Zebrafish beta Catenin Body Patterning Head inhibitor Developmental organiser Gene Expression Regulation Developmental Planarians Wnt/b-catenin signaling Turing-Gierer model for morphogenesis Biological Evolution Intercellular Signaling Peptides and Proteins lcsh:Q RNA Interference Head Signal Transduction |
| Zdroj: | Nature Communications Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019) Nature Communications, Vol. 10, No 312 (2019) |
| ISSN: | 2041-1723 |
| Popis: | Polyps of the cnidarian Hydra maintain their adult anatomy through two developmental organizers, the head organizer located apically and the foot organizer basally. The head organizer is made of two antagonistic cross-reacting components, an activator, driving apical differentiation and an inhibitor, preventing ectopic head formation. Here we characterize the head inhibitor by comparing planarian genes down-regulated when β-catenin is silenced to Hydra genes displaying a graded apical-to-basal expression and an up-regulation during head regeneration. We identify Sp5 as a transcription factor that fulfills the head inhibitor properties: leading to a robust multiheaded phenotype when knocked-down in Hydra, acting as a transcriptional repressor of Wnt3 and positively regulated by Wnt/β-catenin signaling. Hydra and zebrafish Sp5 repress Wnt3 promoter activity while Hydra Sp5 also activates its own expression, likely via β-catenin/TCF interaction. This work identifies Sp5 as a potent feedback loop inhibitor of Wnt/β-catenin signaling, a function conserved across eumetazoan evolution. Hydra regenerate various body parts on amputation by activation of the appropriate organiser, but how head formation is controlled is unclear. Here, the authors identify the transcription factor Sp5 as restricting head formation, by being activated by beta-catenin and then acting as a repressor of Wnt3. |
| Databáze: | OpenAIRE |
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