Pro-osteoclastic in vitro effect of Polyethylene-like nanoparticles: Involvement in the pathogenesis of implant aseptic loosening

Autor: Brulefert, K., Córdova, L.A., Brulin, B., Faucon, A., Hulin, P., Nedellec, S., Gouin, F., Passuti, N., Ishow, E., Heymann, D.
Přispěvatelé: Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre hospitalier universitaire de Nantes (CHU Nantes), Department of Oral and Maxillofacial Surgery [Santiago, Chile], Universidad de Chile = University of Chile [Santiago] (UCHILE)-San Borja Arriarán University Hospital - Faculty of Dentistry [Santiago, Chile], Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Structure fédérative de recherche François Bonamy (SFR François Bonamy), Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Santé de l'Université de Nantes (IRS-UN), Department of Oncology and Metabolism [Sheffield, UK], The University of Sheffield [Sheffield, U.K.], Financial support for this research was provided by the 'Agence Nationale de la Recherche' - GRANT 2007 Pathophysiology of Human Diseases Project N°RO 7196N, INSERM, University of Nantes, University of Chile, CONICYT- Becas Chile and by 'Société Civile de Brevets et Recherches sur les Implants Osseux Hip-Care'., Heymann, Dominique, Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Journal of Biomedical Materials Research Part A
Journal of Biomedical Materials Research Part A, Wiley, 2016, 104 (11), pp.2649-57. ⟨10.1002/jbm.a.35803⟩
Journal of Biomedical Materials Research Part A, 2016, 104 (11), pp.2649-57. ⟨10.1002/jbm.a.35803⟩
ISSN: 1549-3296
1552-4965
DOI: 10.1002/jbm.a.35803⟩
Popis: Polyethylene micro-sized wear particles released from orthopedic implants promote inflammation and osteolysis; however, less is known about the bioactivity of polyethylene nanosized wear particles released from the last generation of polymer-bearing surfaces. We aim to assess the internalization of fluorescent polyethylene-like nanoparticles by both human macrophages and osteoclasts and also, to determine their effects in osteoclastogenesis in vitro. Human macrophages and osteoclasts were incubated with several ratios of fluorescent polyethylene-like nanoparticles between 1 and 72 h, and 4 h, 2, 4, 6, and 9 days, respectively. The internalization of nanoparticles was quantified by flow cytometry and followed by both confocal and video time-lapse microscopy. Osteoclast differentiation and activity was semiquantified by tartrate-resistant acid phosphatase (TRAP) staining, TRAP mRNA relative expression, and pit resorption assay, respectively. Macrophages, osteoclast precursors and mature osteoclasts internalized nanoparticles in a dose- and time-dependent manner and maintained their resorptive activity. In addition, nanoparticles significantly increased the osteoclastogenesis as shown by upregulation of the TRAP expressing cell number. We conclude that polyethylene-like nanosized wear particles promote osteoclast differentiation without alteration of bone resorptive activity of mature osteoclasts and they could be considered as important actors in periprosthetic osteolysis of the last new generation of polymer-bearing surfaces. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2649-2657, 2016.
Databáze: OpenAIRE
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