SNARE proteins play a role in motor axon guidance in vertebrates and invertebrates
| Autor: | Barrecheguren, Pablo José, Ros, Oriol, Cotrufo, Tiziana, Kunz, Beat, Soriano, Eduardo, Ulloa, Fausto, Stoeckli, Esther T, Araújo, Sofia J |
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| Přispěvatelé: | Ministerio de Educación y Ciencia (España), Ministerio de Economía y Competitividad (España), Institute for Research in Biomedicine (Spain), Fundación 'la Caixa', Centro Investigación Biomédica en Red Enfermedades Neurodegenerativas (España), Swiss National Science Foundation, University of Zurich, Ulloa, Fausto |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Motor Neurons
SNARE proteins Axon guidance Neuronal Outgrowth 2804 Cellular and Molecular Neuroscience Chick Embryo Immunohistochemistry 10124 Institute of Molecular Life Sciences Axons Avian Proteins 2806 Developmental Neuroscience Drosophila melanogaster Species Specificity nervous system 570 Life sciences biology Animals Drosophila Proteins Drosophila Syntaxin‐1 Motor axon Syntaxin-1 axon guidance motor axon |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Axonal growth and guidance rely on correct growth cone responses to guidance cues, both in the central nervous system (CNS) and in the periphery. Unlike the signaling cascades that link axonal growth to cytoskeletal dynamics, little is known about the cross‐talk mechanisms between guidance and membrane dynamics and turnover in the axon. Our studies have shown that Netrin‐1/deleted in colorectal cancer signaling triggers exocytosis through the SNARE Syntaxin‐1 (STX‐1) during the formation of commissural pathways. However, limited in vivo evidence is available about the role of SNARE proteins in motor axonal guidance. Here we show that loss‐of‐function of SNARE complex members results in motor axon guidance defects in fly and chick embryos. Knock‐down of Syntaxin‐1, VAMP‐2, and SNAP‐25 leads to abnormalities in the motor axon routes out of the CNS. Our data point to an evolutionarily conserved role of the SNARE complex proteins in motor axon guidance, thereby pinpointing an important function of SNARE proteins in axonal navigation in vivo. Ramón y Cajal Programme . Grant Numbers: RYC‐2007‐00417 , RYC‐2009‐05510 -Ministerio de Educacion y Ciencia . Grant Number: AP2005‐1662 -IRB Barcelona‐la Caixa fellowship -Spanish MINECO . Grant Numbers: SAF2013‐42445R , BFU2010‐21507 -CIBERNED -Swiss National Foundation |
| Databáze: | OpenAIRE |
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