Augmented membrane type 1 matrix metalloproteinase (MT1-MMP):MMP-2 messenger RNA ratio in gastric carcinomas with poor prognosis
| Autor: | Caenazzo, C., Maurizio Onisto, Sartor, L., Scalerta, R., Giraldo, A., Nitti, D., Garbisa, S. |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Tissue Inhibitor of Metalloproteinase-2 Matrix Metalloproteinases Membrane-Associated Reverse Transcriptase Polymerase Chain Reaction Biopsy Metalloendopeptidases Reproducibility of Results Prognosis Urokinase-Type Plasminogen Activator Gelatinases Stomach Neoplasms Humans Matrix Metalloproteinase 2 Female |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1078-0432 |
| Popis: | The activation of zymogen and the amount of proteinase and its inhibition are important in determining the eventual activity of matrix-degrading enzymes involved in tumor aggressiveness. To evaluate a gene complement leading to matrix metalloproteinase 2 (MMP-2; Mr 72,000 gelatinase) activity, membrane type 1 MMP (MT1-MMP), urokinase-type plasminogen activator, MMP-2, and tissue inhibitor of metalloproteinase 2 transcriptional levels were measured in gastric carcinoma biopsies. Comparative tumor:normal tissue reverse transcription-PCR in a cohort of 25 patients revealed up to a 10-fold difference in the expression of MT1-MMP, a metalloproteinase that has been proposed as a membrane receptor activator of MMP-2; a 1-unit increment resulted in a 30% risk to survival. A 20% risk also resulted from a 1-unit increment in the MT1-MMP: MMP-2 ratio, which showed differences of up to 15-fold. Instead, the expression of urokinase-type plasminogen activator, which trips off a cascade ending in the activation of MMP-2, as well as the expression of MMP-2 itself and its inhibitor, tissue inhibitor of metalloproteinase 2, lacked correlation with patient follow-up. Zymography revealed MMP-2 activities that were often in conflict with the transcription results and also with follow-up. The results suggest the evaluation of MT1-MMP and/or MT1-MMP:MMP-2 transcription as a new preoperative molecular-level prognostic factor for gastric carcinoma. |
| Databáze: | OpenAIRE |
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