Detection of serine 473 phosphorylated Akt in acute myeloid leukaemia blasts by flow cytometry
| Autor: | Tazzari, Pl, Cappellini, Alessandra, Grafone, T, Mantovani, I, Ricci, F, Billi, Am, Ottaviani, E, Conte, R, Martinelli, G, Martelli, Am |
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| Přispěvatelé: | TAZZARI PL, CAPPELLINI A, GRAFONE T, MANTOVANI I, RICCI F, BILLI AM, OTTAVIANI E, CONTE R, MARTINELLI G., MARTELLI AM. |
| Rok vydání: | 2004 |
| Předmět: |
acute leukaemia
Blotting Western Sialic Acid Binding Ig-like Lectin 3 prognostic factors Antigens Differentiation Myelomonocytic Protein Serine-Threonine Kinases Flow Cytometry Prognosis protein phosphorylation molecular diagnostics Antigens CD Leukemia Myeloid Proto-Oncogene Proteins Acute Disease Serine Humans Phosphorylation Proto-Oncogene Proteins c-akt flow cytometry |
| Zdroj: | British journal of haematology 126 (2004): 675–681. info:cnr-pdr/source/autori:P.L. TAZZARI, A. CAPPELLINI, T. GRAFONE, I. MANTOVANI, F. RICCI, A.M. BILLI, E. OTTAVIANI, R. CONTE, G. MARTINELLI, A.M. MARTELLI,/titolo:Detection of serine 473 phosphorylated Akt in acute myeloid leukaemia blasts by flow cytometry,/doi:/rivista:British journal of haematology (Print)/anno:2004/pagina_da:675/pagina_a:681/intervallo_pagine:675–681/volume:126 |
| ISSN: | 0007-1048 |
| Popis: | The phosphoinositide 3-kinase/Akt signalling pathway is a recently recognized important parameter in the prognosis and the response to treatment of acute myeloid leukaemia (AML). Akt kinase is activated by phosphorylation on Thr 308 and Ser 473. Active Akt promotes cell growth and survival to apoptotic insults. Thus, it seems important to evaluate Akt phosphorylation in AML blasts. This work aimed to establish whether it was possible to detect Akt phosphorylation on Ser 473 of AML blasts by means of flow cytometry. High levels of Akt activity and phosphorylation were detected in 13 of 15 cases of AML. Flow cytometric analysis revealed similar patterns of Ser 473 expression as was observed with Akt kinase activity and Western blot analysis of Thr 308 and Ser 473 phosphorylation. Double immunostaining enabled the simultaneous flow cytometric detection of an AML-associated antigen (CD33) and Ser 473 phosphorylated Akt in leukaemic blast populations. Our results indicate that flow cytometry enabled the rapid and quantitative assessment of Ser 473 phosphorylated Akt of AML blasts that, when used in combination with cell surface staining, can provide more accurate phenotyping of AML blasts. |
| Databáze: | OpenAIRE |
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