A Subset of Histone H2B Genes Produces Polyadenylated mRNAs under a Variety of Cellular Conditions
| Autor: | Kari, Vijayalakshmi, Karpiuk, Oleksandra, Tieg, Bettina, Kriegs, Malte, Dikomey, Ekkehard, Krebber, Heike, Begus-Nahrmann, Yvonne, Johnsen, Steven A. |
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| Přispěvatelé: | Tian, Bin |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DNA Replication
Cytoplasm Anatomy and Physiology Transcription Genetic Bone and Mineral Metabolism Science Biophysics Gene Expression Bone Marrow Cells Biochemistry Histones Molecular cell biology Histone H2B Genes mRNAs Cellular Conditions Cell Line Tumor Genetics Humans RNA Messenger Bone Biology Musculoskeletal System Osteoblasts Chromosome Biology Stem Cells Mesenchymal Stem Cells Cell Differentiation Genomics Cell Cycle Checkpoints HCT116 Cells Chromatin Up-Regulation Nucleic acids Metabolism RNA processing Polyribosomes RNA Medicine Epigenetics Cellular Types Tumor Suppressor Protein p53 Research Article Developmental Biology |
| Zdroj: | PLoS ONE, Vol 8, Iss 5, p e63745 (2013) PLoS ONE |
| Popis: | Unlike other metazoan mRNAs, replication-dependent histone gene transcripts are not polyadenylated but instead have a conserved stem-loop structure at their 39 end. Our previous work has shown that under certain conditions replicationdependent histone genes can produce alternative transcripts that are polyadenylated at the 39 end and, in some cases, spliced. A number of microarray studies examining the expression of polyadenylated mRNAs identified changes in the levels of histone transcripts e.g. during differentiation and tumorigenesis. However, it remains unknown which histone genes produce polyadenylated transcripts and which conditions regulate this process. In the present study we examined the expression and polyadenylation of the human histone H2B gene complement in various cell lines. We demonstrate that H2B genes display a distinct expression pattern that is varies between different cell lines. Further we show that the fraction of polyadenylated HIST1H2BD and HIST1H2AC transcripts is increased during differentiation of human mesenchymal stem cells (hMSCs) and human fetal osteoblast (hFOB 1.19). Furthermore, we observed an increased fraction of polyadenylated transcripts produced from the histone genes in cells following ionizing radiation. Finally, we show that polyadenylated transcripts are transported to the cytoplasm and found on polyribosomes. Thus, we propose that the production of polyadenylated histone mRNAs from replication-dependent histone genes is a regulated process induced under specific cellular circumstances. Open-Access-Publikationsfonds 2013 peerReviewed |
| Databáze: | OpenAIRE |
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