Hemidesmus indicus (L.) R. Br. extract inhibits the early step of herpes simplex type 1 and type 2 replication
| Autor: | Bonvicini, F., Lianza, M., Mandrone, M., Poli, F., Gentilomi, G. A., FABIANA ANTOGNONI |
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| Přispěvatelé: | Bonvicini, Francesca, Lianza, Mariacaterina, Mandrone, Manuela, Poli, Ferruccio, Gentilomi, Giovanna Angela, Antognoni, Fabiana |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Hemidesmus
Dose-Response Relationship Drug α-glucosidase activity Cell Survival Plant Extracts Herpesvirus 2 Human viruses Hemidesmus indicus (L.) R. Br alpha-Glucosidases Herpesvirus 1 Human Mechanism of action Virus Replication Antiviral Agents Virus attachment prevention Chlorocebus aethiops Animals Glycoside Hydrolase Inhibitors Herpes simplex viru Antiviral activity Vero Cells |
| Zdroj: | Scopus-Elsevier |
| Popis: | Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) cause several clinically relevant syndromes in both adults and neonates. Despite the availability of efficient anti-HSV agents, the search for new therapeutic approaches is highly encouraged due to the increasing drug resistance of virus strains. Medicinal plants represent a source of potential bioactive compounds. In this context we evaluated the anti-herpetic activity of Hemidesmus indicus (L.) R. Br., a plant widely used in traditional Indian medicine. The hydroalcoholic extract prepared from roots was characterized by NMR and HPLC analysis and assayed in vitro by CPE reduction and virus infectivity assays to define its anti-viral effect. The extract's mechanism of action was investigated by virucidal and time-of-addition assays and by in vitro α-glucosidase inhibitory assay. The extract exhibited a remarkable anti-herpetic activity at 100 mg/mL, at non-cytotoxic concentration, through multiple mechanisms: it reduced the infectivity of viral particles released from infected cells possibly through its anti-ER α-glucosidase inhibitory activity and it inhibited the beginning stage of HSV infection acting as a virucide agent and/or preventing virus attachment to the host cell surface. |
| Databáze: | OpenAIRE |
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