Effects of the Staphylococcus aureus and Staphylococcus epidermidis Secretomes Isolated from the Skin Microbiota of Atopic Children on CD4+ T Cell Activation
| Autor: | Laborel-Préneron, Emeline, Bianchi, Pascale, Boralevi, Franck, Lehours, Philippe, Fraysse, Frédérique, Morice-Picard, Fanny, Sugai, Motoyuki, Sato'o, Yusuke, Badiou, Cédric, Lina, Gérard, Schmitt, Anne-Marie, Redoulès, Daniel, Casas, Christiane, Davrinche, Christian |
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| Přispěvatelé: | Institut des Sciences et du Medicament Toulouse, Toulouse, France. (UMR2587-Pierre Fabre), PIERRE FABRE-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Pharmacochimie Pierre Fabre Dermo-Cosmétique, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Hôpital Pellegrin, CHU Bordeaux [Bordeaux]-Groupe hospitalier Pellegrin, Laboratoire de Bactériologie, Université Bordeaux Segalen - Bordeaux 2, Hiroshima University, Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
CD4-Positive T-Lymphocytes Staphylococcus aureus lcsh:R lcsh:Medicine Infant Dermatitis Immunoglobulin E [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Atopic Interleukin-10 Interferon-gamma [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Staphylococcus epidermidis Humans [SDV.IMM]Life Sciences [q-bio]/Immunology lcsh:Q Female lcsh:Science Child Preschool Research Article Skin |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2015, 10 (10), pp.e0141067. ⟨10.1371/journal.pone.0141067⟩ PLoS ONE, Vol 10, Iss 10, p e0141067 (2015) PLoS ONE, 2015, 10 (10), pp.e0141067. ⟨10.1371/journal.pone.0141067⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0141067⟩ |
| Popis: | International audience; Interactions between the immune system and skin bacteria are of major importance in the pathophysiology of atopic dermatitis (AD), yet our understanding of them is limited. From a cohort of very young AD children (1 to 3 years old), sensitized to Dermatophagoides pteronyssinus allergens (Der p), we conducted culturomic analysis of skin microbiota, cutaneous transcript profiling and quantification of anti-Der p CD4+ T cells. This showed that the presence of S. aureus in inflamed skin of AD patients was associated with a high IgE response, increased expression of inflammatory and Th2/Th22 transcripts and the prevalence of a peripheral Th2 anti-Der p response. Monocyte-derived dendritic cells (moDC) exposed to the S. aureus and S. epidermidis secretomes were found to release pro-inflammatory IFN-γ and anti-inflammatory IL-10, respectively. Allogeneic moDC exposed to the S. aureus secretome also induced the proliferation of CD4+ T cells and this effect was counteracted by concurrent exposure to the S. epidermidis secretome. In addition, whereas the S. epidermidis secretome promoted the activity of regulatory T cells (Treg) in suppressing the proliferation of conventional CD4+ T cells, the Treg lost this ability in the presence of the S. aureus secretome. We therefore conclude that S. aureus may cause and promote inflammation in the skin of AD children through concomitant Th2 activation and the silencing of resident Treg cells. Commensals such as S. epidermidis may counteract these effects by inducing the release of IL-10 by skin dendritic cells. |
| Databáze: | OpenAIRE |
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