A Phox2b::FLPo transgenic mouse line suitable for intersectional genetics
| Autor: | Marie-Rose, Hirsch, Fabien, d'Autréaux, Susan M, Dymecki, Jean-François, Brunet, Christo, Goridis |
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| Přispěvatelé: | Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
MESH: Mice
Transgenic MESH: Neurons Muscle Proteins Mice Transgenic MESH: Transgenes MESH: Gene Transfer Techniques Article Mice MESH: Muscle Proteins Mesencephalon MESH: Gene Expression Regulation Developmental MESH: Homeodomain Proteins Animals MESH: Animals Transgenes MESH: Mice [SDV.BDD]Life Sciences [q-bio]/Development Biology MESH: Organ Specificity Homeodomain Proteins Neurons Recombination Genetic MESH: DNA Nucleotidyltransferases Gene Transfer Techniques Gene Expression Regulation Developmental MESH: Rhombencephalon MESH: Transcription Factors MESH: Mesencephalon Rhombencephalon Organ Specificity DNA Nucleotidyltransferases MESH: Recombination Genetic Transcription Factors |
| Zdroj: | Genesis Genesis, Wiley-Blackwell, 2013, 51 (7), pp.506-14. ⟨10.1002/dvg.22393⟩ |
| ISSN: | 1526-954X 1526-968X |
| DOI: | 10.1002/dvg.22393⟩ |
| Popis: | International audience; Phox2b is a transcription factor expressed in the central and peripheral neurons that control cardiovascular, respiratory, and digestive functions and essential for their development. Several populations known or suspected to regulate visceral functions express Phox2b in the developing hindbrain. Extensive cell migration and lack of suitable markers have greatly hampered studying their development. Reasoning that intersectional fate mapping may help to overcome these impediments, we have generated a BAC transgenic mouse line, P2b::FLPo, which expresses codon-optimized FLP recombinase in Phox2b expressing cells. By partnering the P2b::FLPo with the FLP-responsive RC::Fela allele, we show that FLP recombination switches on lineage tracers in the cells that express or have expressed Phox2b, permanently marking them for study across development. Taking advantage of the dual-recombinase feature of RC::Fela, we further show that the P2b::FLPo transgene can be partnered with Lbx1(Cre) as Cre driver to generate triple transgenics in which neurons having a history of both Phox2b and Lbx1 expression are specifically labeled. Hence, the P2b::FLPo line when partnered with a suitable Cre driver provides a tool for tracking and accessing genetically subsets of Phox2b-expressing neuronal populations, which has not been possible by Cre-mediated recombination alone. |
| Databáze: | OpenAIRE |
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