Differential Use of the C-Type Lectins L-SIGN and DC-SIGN for Phlebovirus Endocytosis

Autor: Léger, Psylvia, Tetard, Marilou, Youness, Berthe, Cordes, Nicole, Rouxel, Ronan N, Flamand, Marie, Lozach, Pierre-Yves
Přispěvatelé: Heidelberg University Hospital [Heidelberg], Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Unité de recherche Virologie et Immunologie Moléculaires (VIM), Institut National de la Recherche Agronomique (INRA), Virologie Structurale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Funded by - CellNetworks Research Group - Natural Sciences and Engineering Research Council of Canada. Grant Number: 419538-2012 - Banting Research Foundation, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Virologie Structurale - Structural Virology, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
binding
MESH: Liver/virology
MESH: Cell Adhesion Molecules/metabolism
MESH: Virus Internalization
Receptors
Cell Surface
bunyavirus
MESH: Endothelial Cells/metabolism
DC-SIGN
virus receptor
L-SIGN
MESH: Phlebovirus/pathogenicity
MESH: Endothelial Cells/virology
Humans
endocytosis
MESH: Protein Binding
Lectins
C-Type
C-type lectins
CD209L
phlebovirus
MESH: Receptors
Cell Surface/genetics
MESH: Humans
MESH: Phlebovirus/physiology
MESH: Lectins
C-Type/genetics
Endothelial Cells
Uukuniemi virus
endocytic motif
Virus Internalization
MESH: Liver/cytology
CD209
Rift Valley fever virus
DC-SIGNR
Liver
uptake
MESH: Endocytosis
MESH: HeLa Cells
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
MESH: Receptors
Cell Surface/metabolism
MESH: Cell Adhesion Molecules/genetics
Cell Adhesion Molecules
MESH: Lectins
C-Type/metabolism
HeLa Cells
Protein Binding
Zdroj: Traffic
Traffic, Wiley, 2016, 17 (6), pp.639-56. ⟨10.1111/tra.12393 ⟩
Traffic, Wiley, 2016, 17 (6), pp.639-56. ⟨10.1111/tra.12393⟩
Traffic, 2016, 17 (6), pp.639-56. ⟨10.1111/tra.12393⟩
ISSN: 1398-9219
1600-0854
Popis: International audience; Bunyaviruses represent a growing threat to humans and livestock globally. The receptors, cellular factors and endocytic pathways used by these emerging pathogens to infect cells remain largely unidentified and poorly characterized. DC-SIGN is a C-type lectin highly expressed on dermal dendritic cells that has been found to act as an authentic entry receptor for many phleboviruses (Bunyaviridae), including Rift Valley fever virus (RVFV), Toscana virus (TOSV) and Uukuniemi virus (UUKV). We found that these phleboviruses can exploit another C-type lectin, L-SIGN, for infection. L-SIGN shares 77% sequence homology with DC-SIGN and is expressed on liver sinusoidal endothelial cells. L-SIGN is required for UUKV binding but not for virus internalization. An endocytosis-defective mutant of L-SIGN was still able to mediate virus uptake and infection, indicating that L-SIGN acts as an attachment receptor for phleboviruses rather than an endocytic receptor. Our results point out a fundamental difference in the use of the C-type lectins L-SIGN and DC-SIGN by UUKV to enter cells, although both proteins are closely related in terms of molecular structure and biological function. This study sheds new light on the molecular mechanisms by which phleboviruses target the liver and also highlights the added complexity in virus-receptor interactions beyond attachment.
Databáze: OpenAIRE
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