(13)C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes
| Autor: | Guzmán Avilés, Shirley Doris, Marín Martínez, Silvia, Miranda, Aníbal, Selivanov, Vitaly, Centelles Serra, Josep Joan, Harmancey, Romain, Smih, Fatima, Turkieh, Annie, Durocher, Yves, Zorzano Olarte, Antonio, Rouet, Philippe, Cascante i Serratosa, Marta |
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| Přispěvatelé: | Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona (UB)-Facultat de Biologia, Institute for Research in Biomedicine (IRB), University of Barcelona, Equipe 7 Inserm U1048, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Animal Cell Technology Group, Consiglio Nazionale delle Ricerche [Roma] (CNR)-Biotechnologies Research Institute, Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III [Madrid] (ISC), This study was supported by European Union-funded project ETHERPATHS (FP7-KBBE-222639) and from Agència de Gestió d'Ajuts Universitaris i de Recerca (AGAUR)-Generalitat de Catalunya (2014SGR1017 and 2006ITT-10007), Dupuis, Christine, Universitat de Barcelona |
| Rok vydání: | 2014 |
| Předmět: |
endocrine system diseases
Bioinformatics Muscle Fibers Skeletal [SDV.BC]Life Sciences [q-bio]/Cellular Biology Insulina Cell diferentiation Tracer-based metabolomics Animals Insulin Pyruvate Dehydrogenase (Lipoamide) Resistin [SDV.BC] Life Sciences [q-bio]/Cellular Biology Fluxomics Carbon Isotopes Glucose metabolism nutritional and metabolic diseases Computational Biology Insulin resistance respiratory system Metabolisme Metabolic Flux Analysis Rats Metabolism Glucose Glucosa Diferenciació cel·lular Resistència a la insulina hormones hormone substitutes and hormone antagonists Phosphoenolpyruvate Carboxykinase (ATP) Software Research Article |
| Zdroj: | BMC Systems Biology BMC Systems Biology, BioMed Central, 2014, 8 (1), pp.109. ⟨10.1186/s12918-014-0109-z⟩ BMC Systems Biology, 2014, 8 (1), pp.109. ⟨10.1186/s12918-014-0109-z⟩ Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de la UB Universidad de Barcelona |
| ISSN: | 1752-0509 |
| Popis: | International audience; : BackgroundIt has been suggested that the adipokine resistin links obesity and insulin resistance, although how resistin acts on muscle metabolism is controversial. We aimed to quantitatively analyse the effects of resistin on the glucose metabolic flux profile and on insulin response in L6E9 myotubes at the metabolic level using a tracer-based metabolomic approach and our in-house developed software, Isodyn.ResultsResistin significantly increased glucose uptake and glycolysis, altering pyruvate utilisation by the cell. In the presence of resistin, insulin only slightly increased glucose uptake and glycolysis, and did not alter the flux profile around pyruvate induced by resistin. Resistin prevented the increase in gene expression in pyruvate dehydrogenase-E1 and the sharp decrease in gene expression in cytosolic phosphoenolpyruvate carboxykinase-1 induced by insulin.ConclusionsThese data suggest that resistin impairs the metabolic activation of insulin. This impairment cannot be explained by the activity of a single enzyme, but instead due to reorganisation of the whole metabolic flux distribution. |
| Databáze: | OpenAIRE |
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